Abstract: The invention relates to a system (1) for supplying a microfluidic subsystem with liquids, comprising a first valve (14, 29, 46) and a first fluidic duct (10, 25, 28), for connecting said first valve (14, 29, 46) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct (11), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve (14, 29, 46) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct (10, 15, 28) are chosen such that the value of X1[Pa?1], defined as: X1[Pa?1]=(0.

Abstract: The invention relates to a method for splitting droplets on demand in a microfluidic junction, comprising the supply channel, the first drain channel and the second drain channel, the method comprises the following steps: a. delivering a droplet (1) to the said microfluidic junction (3) through said supply channel (2) by means of a flow of continuous liquid through the supply channel (2) and said first drain channel, b. stopping the flow in said first drain channel and opening the flow in said second drain channel until a fraction (7) of the droplet (1) is present in the second drain channel, c. resuming the flow in the first drain channel and closing the flow in the second drain channel, at least until the fraction (7) of the said droplet (1) being present in the first drain channel separates from the rest of the droplet.

Abstract: The invention relates to a system (1) for supplying a microfluidic subsystem with liquids, comprising a first valve (14, 29, 46) and a first fluidic duct (10, 25, 28), for connecting said first valve (14, 29, 46) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct (11), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve (14, 29, 46) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct (10, 15, 28) are chosen such that the value of X1[Pa?1], defined as: X1[Pa?1]=(0.