Abstract: The disclosed invention relates to a reaction bottle comprising a container with a container opening and a container interior, a septum associated with the container and configured to releasably seal the container opening, a needle holder associated with the container, the needle holder defining a holder cavity, a needle associated with the needle holder, the needle disposed at least partially within the holder cavity, wherein the septum is deformable between a sealing rest state and a punctured state, and the septum is deformable into puncturable impingement with an end of the needle when the septum is in the punctured state.