Abstract: The present invention describes, in certain embodiments, a composition for detecting a tau protein comprising a modified detector molecule having two ends, a first end capable of binding the tau protein and a second end comprising a single-stranded DNA template, wherein the template is capable of being replicated by an RNA polymerase.

Abstract: Described herein are compositions and methods for identifying cellular factors involved in protein agglomeration. One such factor is a nucleic acid component. Another factor is a cellular binding factor. The nucleic acid components, and methods of using them, to interact with agglomeration proteins are also disclosed herein. The nucleic acid compositions herein comprise one or more DNA or RNA molecules having affinity for at least one agglomeration protein. The nucleic acid component is a naturally or non-naturally occurring molecule with twenty or more ribonucleotide bases. For RNA, at least one nucleotide sequence portion of this RNA molecule has affinity to at least one consensus sequence present in the agglomeration RNA-binding protein. Methods disclosed herein are directed towards detecting the presence of one or more agglomeration proteins in a sample matrix using the amplibody compositions described herein.

Abstract: Embodiments of the present invention feature compositions and methods for making an animal and an animal exhibiting characteristics of an agglomeration disease. The method comprises the steps of providing an animal cell and placing a vector in said cell having a non-coding RNA under the control of a promoter and controlling the expression of said non-coding RNA with said promoter.

Abstract: The present invention describes, in certain embodiments, a composition for detecting a tau protein comprising a modified detector molecule having two ends, a first end capable of binding the tau protein and a second end comprising a single-stranded DNA template, wherein the template is capable of being replicated by an RNA polymerase.

Abstract: Embodiments of the present invention are directed to methods and kits for the generation of double stranded RNA by RNA dependent RNA polymerases. The methods and kits feature exponential generation of such RNAs through simple steps/ The RNA is suitable for interference with expression in cells.

Abstract: Disclosed herein are methods of detecting target molecules from a complex sample using a modified affinity ligand. In particular, the modified affinity ligand is an antibody specific for the target and has conjugated to it a nucleic acid molecule. This conjugated nucleic acid molecule serves as a template for a replicase that can replicate the template a billion-fold in an matter of minutes. The replication products then serve to facilitate a detectable signal indicating the presence of the target in the sample.

Abstract: The present invention is directed to methods, compositions, kits and devices for making a nucleic acid having selected affinity to a target molecule. One embodiment of the present invention is a method of making a replicatable nucleic acid template having a selected affinity to a target. The method comprises the step of applying a selection to a first generation comprising at least one replicatable nucleic acid template as the replicatable nucleic acid template is replicated by a nucleic acid polymerase to form at least one subsequent generation comprising a replicatable nucleic acid template. The selection is based on the affinity of the replicatable nucleic template of different generations to the target. The nucleic acid polymerase introduces genetic variability between generations of the replicatable nucleic acid template to produce replicatable nucleic acid templates having different affinities to the target.

Abstract: Disclosed herein are methods of detecting target molecules from a complex sample using a modified affinity ligand. In particular, the modified affinity ligand is an antibody specific for the target and has conjugated to it a nucleic acid molecule. This conjugated nucleic acid molecule serves as a template for a replicase that can replicate the template a billion-fold in an matter of minutes. The replication products then serve to facilitate a detectable signal indicating the presence of the target in the sample.

Abstract: The present invention is directed to methods, compositions, kits and apparatus to identify and detect the presence or absence of target analytes. The embodiments of the present invention have utility in medical diagnosis and analysis of various chemical compounds in specimens and samples, as well as the design of test kits and apparatus for implementing such methods.

Abstract: The present invention is directed to methods, compositions, kits and apparatus to identify and detect the presence or absence of target analytes. The embodiments of the present invention have utility in medical diagnosis and analysis of various chemical compounds in specimens and samples, as well as the design of test kits and apparatus for implementing such methods.

Abstract: The present invention is directed to methods, compositions, kits and devices for making a nucleic acid having selected affinity to a target molecule. One embodiment of the present invention is a method of making a replicatable nucleic acid template having a selected affinity to a target. The method comprises the step of applying a selection to a first generation comprising at least one replicatable nucleic acid template as the replicatable nucleic acid template is replicated by a nucleic acid polymerase to form at least one subsequent generation comprising a replicatable nucleic acid template. The selection is based on the affinity of the replicatable nucleic template of different generations to the target. The nucleic acid polymerase introduces genetic variability between generations of the replicatable nucleic acid template to produce replicatable nucleic acid templates having different affinities to the target.