Abstract: An assay substrate including a first component comprising a sensor molecule labeled with a quantum dot, the quantum dot immobilized to an assay substrate surface with a first linker being a bi-polar linker comprising a first binding group for specific binding of the quantum dot and a second binding group for specific binding of the assay substrate surface, the sensor molecule having a specific binding site for an organic analyte, the sensor molecule labeled with the quantum dot in a position that has no effect on the organic analyte binding the specific binding site; and a second component comprising a chemical analogue of the organic analyte, the chemical analogue labeled with a fluorescent dye, the chemical analogue linked to the quantum dot with a second linker having a length exceeding Foster radius, and the chemical analogue reversibly binding the specific binding site of the sensor molecule of the first component.

Abstract: A method and a device for detection and quantification of various organic analytes in a liquid sample and an assay substrate for providing analyte measurements. The method is implemented by using specific interaction of organic analytes with selective binding sites immobilized on a multi-layer assay substrate with further detection of such interaction with a device based on inducing and recording the fluorescence of the substrate for bio-chemical, genetic and environmental analyses.