Abstract: A method for determining a target nucleic acid sequence is disclosed, wherein a preparation having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, is contacted with a blocking oligonucleotide complementary to at least a portion of the first region of dissimilar sequence of the non-target nucleic acid sequence, under conditions to hybridise the blocking oligonucleotide thereto and hybridized to the target nucleic acid sequence; and then sequenced, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, whereby at least the second region of dissimilar sequence of the target nucleic acid sequence is determined; and wherein the sequencing reaction is blocked at least from proceeding into the second region of dissimilar sequence of the non-target nucleic acid sequence.

Abstract: The present invention relates to an improved method for isolating nucleic acids, particularly genomic desoxyribonucleic acid (DNA) from blood.

Abstract: A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: (a) contacting the preparation with an oligonucleotide primer complementary to at least a portion of the first region of common sequence, under conditions to hybridise the primer thereto; and (b) subjecting the preparation to a sequencing reaction, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, thereby determining at least the second region of dissimilar sequence of the target nucleic acid sequence; and wherein the method further comprises a step of blocking the sequencing reaction between the primer and the non-targe