Patents Assigned to QIAGEN AS
  • Thermostable chimeric nucleic acid polymerases and uses thereof
    Patent number: 8637288
    Abstract: Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.
    Type: Grant
    Filed: August 8, 2002
    Date of Patent: January 28, 2014
    Assignee: Qiagen, GmbH
    Inventors: Dirk Löffert, Andreas Missel, Jie Kang
  • Quantitative helicase assay
    Patent number: 8628914
    Abstract: Disclosed herein are methods and kits relating to detection and quantitation of helicase activity.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: January 14, 2014
    Assignee: QIAGEN Gaithersburg, Inc.
    Inventors: Nadia Allen, Peter Qiu
  • Method for isolating and purifying nucleic acids
    Patent number: 8624020
    Abstract: The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials, using a wash buffer comprising an alcohol having 1 to 3 carbon atoms and at least one further solvent selected from the group consisting of alkane diols and alkane triols having 2 to 6 carbon atoms, monocarboxylic acid esters and dicarboxylic acid diesters having 2 to 6 carbon atoms in the acidic component and 1 to 4 carbon atoms in the alcoholic component; (poly)ethylene glycols and ether derivatives and ester derivatives thereof, and poly(4-styrene sulfonic acid-co-maleic acid) sodium salt solution. The present invention further relates to a kit for carrying out the method of the invention.
    Type: Grant
    Filed: September 2, 2009
    Date of Patent: January 7, 2014
    Assignee: Qiagen GmbH
    Inventors: Ralf Himmelreich, Sabine Werner
  • Capillary electrophoresis using clear coated capillary tubes
    Patent number: 8617374
    Abstract: A capillary tube having a hard, optically clear external coating or cladding. In one embodiment, the external clear coating comprises hard-fluoropolymer. The hard-fluoropolymer coating bonds to the fused silica glass, providing higher strength and superior static fatigue performance resulting in vastly improved bending flexibility. The thin hard-fluoropolymer coating of capillaries provides higher initial tensile strength, longer lifetime (resistance to stress corrosion or static fatigue) and superior ability to transmit excitation light and emitted light directly through the coating for fluorescence based detection.
    Type: Grant
    Filed: March 26, 2008
    Date of Patent: December 31, 2013
    Assignee: Qiagen Sciences, LLC
    Inventor: Varouj Amirkhanian
  • Combination of fluorescent dyes for the detection of nucleic acids
    Patent number: 8614061
    Abstract: The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY-632 and DY-520XL.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: December 24, 2013
    Assignee: Qiagen, GmbH
    Inventors: Werner Brabetz, Cornelia Weber
  • POLYNUCLEOTIDE PRIMERS
    Publication number: 20130323736
    Abstract: A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.
    Type: Application
    Filed: March 12, 2013
    Publication date: December 5, 2013
    Applicant: QIAGEN MANCHESTER LIMITED
    Inventor: QIAGEN MANCHESTER LIMITED
  • QUANTIFYING NUCLEIC ACID IN SAMPLES
    Publication number: 20130320223
    Abstract: A photometric device (18) for quantifying a nucleic acid in a sample (518) in a tube (58), comprises a light emission unit (28), a sample obtaining unit (38) for holding the tube (58) with the nucleic acid sample (518) and a detection unit (48) wherein the sample obtaining unit (38) is arranged in between the light emission unit (28) and the detection unit (48) in particular, the light emission unit (28) and the detection unit (48) are arranged to provide light through the sample obtaining unit (38) such that light of a first wavelength of about 230 nanometers and light of a second wavelength of about 260 nanometers are simultaneously detectable within the detection unit (48). The photometric device (18) allows for efficiently analysing the nucleic acid sample by only considering invisible light.
    Type: Application
    Filed: November 23, 2011
    Publication date: December 5, 2013
    Applicant: QIAGEN LAKE CONSTANCE GMBH
    Inventors: Harald Quintel, Elmar Christian Feuerbacher, Roman Gruler, Stuart Ronald Huckins, Dominik Zahr, Markus Rebholz, Robert Schild
  • Compositions and methods for using a solid support to purify DNA
    Patent number: 8598338
    Abstract: The present disclosure provides methods for isolating substantially pure and undegraded DNA from biological material via a solid support. The methods may use a DNA lysing solution that comprises a surfactant, a DNA-complexing salt (e.g., a lithium salt), and a buffer. Alternatively, the methods may use a solid support pre-treated with the DNA lysing solution.
    Type: Grant
    Filed: March 5, 2010
    Date of Patent: December 3, 2013
    Assignee: QIAGEN North American Holdings, Inc.
    Inventors: Robert Jackson Bair, Kristen Campbell Benedict, Wendy J. Kivens, Robert W. Kwiatkowski, Jr., Kim Paulsen, Daniel A. Strom, John M. Wages
  • Optical detection alignment coupling apparatus
    Patent number: 8597485
    Abstract: An apparatus for aligning a capillary column with one or more excitation fibers and with one or more optical lens elements for Capillary Electrophoresis. The apparatus includes two identical blocks having a plurality of grooves for positioning and aligning the capillary column with the one or more excitation fibers, and a plurality of lens seats for optically coupling the lens element with the capillary column. Each block includes a male and female part for mating the two identical blocks together.
    Type: Grant
    Filed: April 5, 2010
    Date of Patent: December 3, 2013
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Paul Mooney
  • System for providing control reactions for real time RT-PCR
    Patent number: 8597938
    Abstract: Provided are methods and oligonucleotides useful as primers and templates for internal controls designed for use in Real Time Reverse Transcriptase Polymerase Chain Reactions. Use of the present methods and oligonucleotides allows validation of assay parameters and of the results that an assay set.
    Type: Grant
    Filed: October 10, 2008
    Date of Patent: December 3, 2013
    Assignee: QIAGEN Sciences LLC
    Inventors: Jingping Yang, Li Shen
  • FLUID PROCESSING TUBE FOR OPTICAL ANALYSIS AND METHOD FOR ANALYZING A FLUID
    Publication number: 20130314697
    Abstract: Fluid processing tube for use in optical analysis comprising at least one first portion being made from a first material suitable for optical analysis and being configured to include two optical paths of different lengths, and at least one second portion connected to said first portion and being made from a second material different from said first material.
    Type: Application
    Filed: November 23, 2011
    Publication date: November 28, 2013
    Applicant: Qiagen GmbH
    Inventors: Thomas Voit, Michael Geissler, Harald Quintel, Roman Gruler
  • MAGNETIC MICROPLATE ASSEMBLY
    Publication number: 20130315800
    Abstract: Magnetic microplate assemblies are disclosed for assaying of biological activated magnetic particles from a supernatant. The assemblies include a magnetic microplate holder and a microplate including a plurality of wells for retaining the magnetic particles. The microplate holder includes a plurality of magnets for attracting the magnetic particles to the wells and a plurality of detents for securing the microplate in the holder. The detents allow the microplate to be tightly retained in the holder when the assembly is turned over to discard supernatant and wash buffer applied to rinse supernatant from the magnetic particles.
    Type: Application
    Filed: March 15, 2013
    Publication date: November 28, 2013
    Applicant: QIAGEN GAITHERSBURG, INC.
    Inventors: Fei YIN, Bandele JEFFREY-COKER
  • Method for detecting and quantifying poly(A) RNA and mRNA
    Patent number: 8586304
    Abstract: The present invention relates to a method for detecting and/or for quantifying poly(A) RNA and/or mRNA, wherein a poly(dT) oligonucleotide which features a fluorescent dye and also a quencher is hybridized to the nucleic acid to be detected. Non-hybridized poly(dT) oligonucleotide is degraded by an added nuclease, and as a result, fluorescently labelled nucleotides are released and the resulting fluorescent signal is measured.
    Type: Grant
    Filed: December 1, 2010
    Date of Patent: November 19, 2013
    Assignee: Qiagen GmbH
    Inventor: Nan Fang
  • Method and device for breaking down biological material
    Patent number: 8573518
    Abstract: The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.
    Type: Grant
    Filed: April 24, 2009
    Date of Patent: November 5, 2013
    Assignee: QIAGEN, GmbH
    Inventors: Thomas Rothmann, Claudia Schmid, Andreas Schafer, Gabriele Christoffel
  • EXTRACTION OF NUCLEIC ACIDS FROM WAX-EMBEDDED SAMPLES
    Publication number: 20130280787
    Abstract: The present invention relates to a method for extracting nucleic acids from a wax-embedded sample, the use of particular solvents for removing wax from a wax-embedded sample in a method for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample as well as to a kit for extracting, isolating and/or purifying nucleic acids from a wax-embedded sample.
    Type: Application
    Filed: June 14, 2011
    Publication date: October 24, 2013
    Applicant: QIAGEN GmbH
    Inventors: Markus Mueller, Thorsten Singer, Esther Rosenberg, Joerg Hucklenbroich
  • METHOD AND KIT FOR PROCESSING WAX-EMBEDDED BIOLOGICAL SAMPLES
    Publication number: 20130280728
    Abstract: The present invention relates to a method for processing a wax-embedded biological sample, the use of poly(organosiloxane)s for liquefying the embedding medium of a wax-embedded biological sample and a kit for processing a wax-embedded biological sample.
    Type: Application
    Filed: December 23, 2011
    Publication date: October 24, 2013
    Applicant: QIAGEN GMBH
    Inventor: Martin Schlumpberger
  • Permanent inactivation of nucleases
    Patent number: 8563262
    Abstract: The present invention relates to the use of reagent for permanently inactivating nucleases wherein the reagent comprises an oxidizing agent, a protein denaturant and optionally a pH adjustor and to a method for permanently inactivating nucleases using said reagent.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: October 22, 2013
    Assignee: Qiagen GmbH
    Inventors: Gabriele Christoffel, Martin Schlumpberger, Heike Glowatz
  • Sample holder and method of using the same
    Patent number: 8563326
    Abstract: A sample holder, such as a microscope slide, is provided in the form of a card-shaped substrate or plate, preferably for use in an analytical reader. The sample holder comprises at least one hole, preferably a plurality of holes, for receiving a sample to be analyzed. The at least one hole extends completely through the substrate and is sized such that the sample is held within the at least one hole by means of the surface tension of the sample against the force of gravity. Optionally the substrate comprises a first upper substrate and a second lower substrate that together embed a porous membrane. As a further option the sample holder comprises a first cover attached to the top side of the first upper substrate and/or a second cover attached to the bottom side of the second lower substrate.
    Type: Grant
    Filed: March 31, 2009
    Date of Patent: October 22, 2013
    Assignee: Qiagen Lake Constance GmbH
    Inventors: Klaus Haberstroh, Konrad Faulstich
  • Detection of nucleic acids by target-specific hybrid capture method
    Patent number: 8557973
    Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods.
    Type: Grant
    Filed: October 14, 2010
    Date of Patent: October 15, 2013
    Assignee: Qiagen Gaithersburg, Inc.
    Inventors: James Anthony, Attila Lorincz, John Troy, Yanglin Tan
  • Rapid method for identifying polypeptide-nucleic acid interactions
    Patent number: 8536100
    Abstract: The invention relates to a method for analysing interactions of a nucleic acid with at least one polypeptide, comprising (a) providing a reaction vessel with a composition comprising cross-linked complexes comprising at least one nucleic acid and at least one polypeptide; (b) immobilizing at least part of the cross-linked complexes to the reaction vessel; (c) at least partially reversing said cross-links in said immobilized complexes by performing a heat incubation step thereby at least partially releasing the nucleic acids from the complexes; (d) analyzing the released nucleic acid. wherein steps (a) to (d) are performed in one reaction vessel. Also provided are specific reaction vessels and kits.
    Type: Grant
    Filed: March 13, 2009
    Date of Patent: September 17, 2013
    Assignee: Qiagen GmbH
    Inventor: Franz Narz