Patents Assigned to QIAGEN MANCHESTER LIMITED
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Patent number: 10196692Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: September 18, 2015Date of Patent: February 5, 2019Assignee: QIAGEN MANCHESTER LIMITEDInventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Patent number: 10190171Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: September 18, 2015Date of Patent: January 29, 2019Assignee: QIAGEN MANCHESTER LIMITEDInventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Patent number: 9863003Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: October 24, 2013Date of Patent: January 9, 2018Assignee: QIAGEN MANCHESTER LIMITEDInventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Patent number: 9328385Abstract: The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.Type: GrantFiled: February 20, 2012Date of Patent: May 3, 2016Assignees: Qiagen GmbH, Qiagen Manchester LimitedInventors: Francesca Di Pasquale, Holger Engel, Sascha Strauss, Nicola Jo Thelwell
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Patent number: 9273350Abstract: A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerisation thereof, under conditions such that the template binding region of the primer will hybridise to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product from the template whereupon the target binding region in the tail of the primer will hybridise to a sequence in the primer extension product corresponding to the target nucleic acid, and wherein any such target specific hybridisation causes a detectable change in the signalling system, such that the presence or absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a deType: GrantFiled: June 8, 2004Date of Patent: March 1, 2016Assignee: QIAGEN MANCHESTER LIMITEDInventors: David Mark Whitcombe, Jane Theaker, Neil James Gibson, Stephen Little
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Publication number: 20150211076Abstract: A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.Type: ApplicationFiled: April 9, 2015Publication date: July 30, 2015Applicant: QIAGEN MANCHESTER LIMITEDInventors: David Mark WHITCOMBE, Nicola Jo THELWELL, Paul Francis RAVETTO
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Patent number: 9029084Abstract: A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.Type: GrantFiled: April 4, 2006Date of Patent: May 12, 2015Assignee: Qiagen Manchester LimitedInventors: David Mark Whitcombe, Nicola Jo Thelwell, Paul Francis Ravetto
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Patent number: 8901285Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: September 29, 2008Date of Patent: December 2, 2014Assignee: Qiagen Manchester LimitedInventors: Ruth Board, Jennifer Ferguson, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Publication number: 20140147843Abstract: The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.Type: ApplicationFiled: February 20, 2012Publication date: May 29, 2014Applicants: QIAGEN MANCHESTER LIMITED, QIAGEN GMBHInventors: Francesca Di Pasquale, Holger Engel, Sascha Strauss, Nicola Jo Thel Well
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Publication number: 20140141425Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: ApplicationFiled: October 24, 2013Publication date: May 22, 2014Applicant: QIAGEN MANCHESTER LIMITEDInventors: RUTH BOARD, JENNIFER FERGUSON, PAUL FRANCIS RAVETTO, NICOLA JO THELWELL, DAVID WHITCOMBE
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Publication number: 20130323736Abstract: A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.Type: ApplicationFiled: March 12, 2013Publication date: December 5, 2013Applicant: QIAGEN MANCHESTER LIMITEDInventor: QIAGEN MANCHESTER LIMITED