Abstract: The present disclosure provides methods, sets of substantially complementary double-stranded adapters, and kits for performing nucleic acid sequencing. The substantial complementary double-stranded adapters comprise fully complementary molecular tag regions but one or more mismatches in other regions. Such adapters are ligated to double-stranded target nucleic acids, the obtained ligation products are amplified, and the generated amplification products are sequenced. The methods according to the present disclosure allow both strands of double-stranded target nucleic acids to be sequenced from one end of the target nucleic acids.
Abstract: Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.
Type:
Grant
Filed:
September 2, 2016
Date of Patent:
February 8, 2022
Assignee:
QIAGEN Sciences, LLP
Inventors:
Heather Callahan, Eddie Adams, Mark N. Brolaski, Victoria Nieciecki