Abstract: A lithium-ion battery separator with high-temperature resistance, a preparation method thereof and a lithium-ion battery prepared therefrom fall within the field of lithium-ion battery separators. The separator has a thickness of 3.5-30 ?m, a porosity of 30-80%, an adjustable pore size of 20-2000 nm, a biaxial tensile strength of ?50 MPa, an air permeability of ?400 s/100 cc, and a breaking temperature of ?160° C. The preparation method comprises the following steps: mixing, melting, and plasticizing 20%-60% of a polypropylene main material, 2%-10% of a solubilizer, 30%-80% of a solvent. 0.1%-5% of a nucleating aid and/or 0.1%-1% of an antioxidant, carrying out twin-screw extrusion, carrying out thermally induced phase separation to obtain a cast sheet, and carrying out cast sheet stretching, extraction, and post-treatment or directly carrying out extraction and post-treatment.