Abstract: Substrate for a microfluidic device, including at least one microfluidic structure having at least one access port at an upper surface of the substrate, a raised support structure positioned on the upper surface adjacent to each access port and surrounding the access port, the raised support structure partially covering the substrate upper surface, the first raised support structure having an upper surface for receiving an adhesive for mounting a microfluidic component having at least one access port corresponding to the at least one access port of the substrate. A microfluidic device, including a substrate, a microfluidic component having at least one access port at a lower surface corresponding to the at least one access port of the substrate. The microfluidic component is mounted on the top of the substrate with an adhesive applied between the upper surface of the at least one first and/or second raised support structure and the lower surface of the microfluidic component.