Abstract: Methods and compositions are provided for improving the production of products, such as fuel products like ethanol, in microorganisms. In particular, methods and compositions are described for improving ethanol production utilizing genes identified in Clostridium phytofermentans.

Abstract: Improved yields of biofuels and other chemicals are obtained by culturing a cellulolytic microorganism, such as Clostridium phytofermentans, Clostridium sp. Q.D, or Clostridium biocatalysts thereof in modulated amounts of vitamins, such as thiamine or nicotinic acid. Provided are methods to increase yields of ethanol and other fermentation products through vitamin supplementation. Recombinant microorganisms with altered metabolic pathways that obviate the need to modulate media components during hydrolysis and fermentation of biomass are also described.

Abstract: Provided are isolated novel Clostridium phytofermentans biocatalysts with deregulated cellulase activity that produce high yields of products. Further provided are methods of using the biocatalysts to degrade organic material and for use in industrial processes.

Abstract: Compositions and methods are provided for redirecting metabolic solventogenesis pathways to enhance the product yield from fermentation of biomass. Clostridium microorganism pathways are modified to extend the growth phase and prevent inhibition of acetaldehyde while bypassing the synthesis of acetyl CoA.

Abstract: This invention provides systems and methods for the increased production of ethanol and other chemical compounds by recombinant Clostridium species whereby the recombinant species are genetically-engineered to disrupt lactate dehydrogenase activity and to hydrolyze and ferment carbonaceous biomass and synthesize compounds of commercial value without production of lactic acid.

Abstract: This invention provides systems and methods for the production of compounds by C. phytofermentans. C. phytofermentans is genetically-engineered for hydrolysis and fermentation of carbonaceous biomass to synthesize compounds of commercial value.

Abstract: Methods and compositions are provided for improving the production of products, such as fuel products like ethanol, in microorganisms. In particular, methods and compositions are described for improving ethanol production utilizing genes identified in Clostridium phytofermentans.

Abstract: In one aspect, methods to enhance the production of ethanol and other fermentive end products from a wide variety of feedstocks by Clostridium microorganisms, such as Clostridium phytofermentans are disclosed. A method of improving fermentation performance of Clostridium microorganisms, such as Clostridium phytofermentans through the use of a fed-batch strategy is described, as well as methods of producing fermentive end products, such as alcohols and/or chemicals by fermenting Clostridium microorganisms, such as Clostridium phytofermentans in the presence of fatty acid-containing compounds and/or at reduced pH.

Abstract: In one aspect, this invention relates to production of useful fermentation end-products from biomass through simultaneous hydrolysis and fermentation by a microorganism, such as Clostridium phytofermentans. The invention also relates to the development of a process for efficient pretreatment and conversion of lignocellulosic biomass to end-products with high conversion efficiency (yield). In another aspect, methods for producing a fermentation end-product by fermenting hexose (C6) and pentose (C5) sugars with a microorganism, such as Clostridium phytofermentans are disclosed herein.

Abstract: Compositions and methods are provided for enhancing saccharification of biomass with one or more enzymes to enhance availability of substrates for fermentation by a microorganism. Microorganisms are also modified to enhance activity of one or more hydrolytic enzymes that are present endogenously in or are introduced heterologously into a host microorganism.