Abstract: This invention relates to a rapid method for detection and characterization of Escherichia coli bacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect E. coli O157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
Type:
Grant
Filed:
February 18, 2014
Date of Patent:
June 19, 2018
Assignee:
Qualicon Diagnostics LLC
Inventors:
Daniel R. Demarco, Mark A. Jensen, Stephen Varkey