Abstract: The present invention provides apparatus, kits and methods for evaluating binding between one or more binding molecules (e.g., a protein) and one or more ligands. The invention also provides apparatus, kits and methods for detecting and/or quantifying a binding molecule, for example, a protein. Also provided are apparatus, kits and methods for “stripping” a complex biological matrix of low molecular weight components. The invention can be carried out on a smaller process scale, and therefore be more efficient, than previously known methods. The present invention is particularly suitable for use in high-throughput assays, which can be partially or completely automated.