Abstract: The present invention provides a device for concentrating a blood fraction. The device includes an ultrafiltration unit connected to a fluid delivery system for delivering the blood fraction to be concentrated into the ultrafiltration unit. The device also includes a purge fluid delivery system for expelling concentrate from the ultrafiltration unit. In one embodiment, the device accommodates multiple cycles of concentration. A blood fraction concentration method is also provided. The device and method of the present invention are particularly useful for preparing a plasma concentrate suitable for use in a coagulum-based wound sealant.

Abstract: The present invention provides a preparation and application device useful for mixing and dispensing a plurality of fluids, wherein the fluids must be mixed immediately prior to use. In one embodiment, the device includes two fluid delivery systems connected to a manifold having two separate fluid channels. Each fluid channel conducts fluid to a mixing chamber, from which, following mixing, the mixture of fluids is dispensed. The manifold additionally includes a gas channel having an outlet connected to one of the fluid channels, which can be used to supply a pressurized gas to the mixing chamber to expel the mixture from the chamber, reducing the likelihood that the mixture can clog the device. The invention also provides a method for preparing and dispensing a mixture of two fluids.

Abstract: A plasma-buffy coat concentrate that comprises plasma, platelets at a concentration of at least 1.0.times.10.sup.9 cells/ml, and fibrinogen at concentration of at least 5 mg/ml is described. The plasma-buffy coat concentrate can be combined with a fibrinogen activator to form a platelet glue wound sealant. A method for processing blood to produce the plasma-buffy coat concentrate is also provided. The method comprises centrifuging anticoagulated blood to remove red blood cells and produce a plasma-buffy coat mixture. Water is removed from the mixture to produce the plasma-buffy coat concentrate. A fibrinogen activator is mixed with the plasma-buffy coat concentrate to produce a wound sealant, which can then be applied to a wound to facilitate sealing and healing of the wound.