Abstract: The present invention belongs to the technical field of biomedicine, and mainly relates to a miR-7-5p mimic for inhibiting migration and invasion of breast cancer, a screening method and an application thereof. The sequence of the miR-7-5p is shown in SEQ ID NO.1. The present invention has found that the miR-7-5p mimic generates a significant inhibitory effect on breast cancer through targeted inhibition of the molecular mechanism of RYK. In vitro culture system, the miR-7-5p mimic can function to inhibit migration and invasion capabilities of breast cancer. The miR-7-5p mimic can inhibit RYK protein and mRNA levels in breast cancer. In nude mice, the miR-7-5p mimic can also significantly inhibit migration and invasion capabilities of breast cancer. Therefore, the present invention demonstrates that the miR-7-5p mimic can be a small-nucleic-acid drug to significantly inhibit breast cancer metastasis.

Abstract: Preparations of a highly coherent diamond nitrogen vacancy (NV?) and a diamond anvil are provided. A graphite is used as a carbon source, a diamond is used as a crystal seed, aluminum/titanium is used as a nitrogen remover, and a single crystal diamond is synthesized under a high temperature and a high pressure, and high-pressure-high-temperature (HPHT) annealing is performed on the synthesized diamond; after the annealing, multiple NV?s are generated in <100> and <311> crystal orientation growth regions from scratch, while native NV?s in a <111> crystal orientation growth region are disappeared; and the <100> and <311> crystal orientation growth regions do not contain defects related to ferromagnetic elements. The high-density and highly coherent NV?s are produced under nondestructive conditions, and the diamond anvil with controlled NV? depths are prepared to achieve a precise detection of the NV? at a pressure above 60 GPa.

Abstract: The present disclosure relates to a curved conformal frequency selective surface (FSS) radome. The radome includes a dielectric radome and a curved conformal FSS array arranged on an outer wall of the dielectric radome, where the dielectric radome includes a dome, a circular truncated cone and a hollow cylinder which are integrally formed from top to bottom, and the curved conformal FSS array is formed by periodically arraying foldable FSS units on an outer surface of the dielectric radome, the foldable FSS unit being of an axially symmetrical and centrally symmetrical gap structure, and having an overall shape consisting of foldable gaps on a left side, an upper side, a right side and a lower side, the four foldable gaps being sequentially connected in a square shape, and remaining parts of the foldable FSS unit except for the four foldable gaps being all metal patches.

Abstract: The present disclosure belongs to the technical field of biomedicine, and in particular relates to a graphene oxide (GO)-based composite nanoparticle drug delivery system for treating cervical cancer and a preparation method thereof. The composite nanoparticle drug delivery system includes an aptamer NH2-AS1411 (Aptamer NH2-AS1411, APT), monolayer graphene oxide (GO), chitosan oligosaccharide (CO) and ?-polyglutamic acid (?-PGA).

Abstract: A copper ion-complexed poly gamma-glutamic acid (?-PGA)/chitosan (CS)/cotton blended antibacterial knitted fabric and a preparation method includes chitosan that is crosslinked with poly gamma-glutamic acid, then a copper-ammonia complex ion solution is added to prepare a spinning solution. The spinning solution is wet spun and then stretched, washed with water, finished, washed with water, and dried to get copper ion-complexed poly gamma-glutamic acid/chitosan composite fibers. The blended antibacterial knitted fabric is then prepared by using cotton fiber yarns and the composite fibers. There is a very high coordination coefficient between carboxyl groups of gamma-PGA and amino groups of CS, so the structure is stable. Poly-gamma glutamic acid can be used as water-retaining agent and heavy metal ion adsorbent, which can increase the loading rate of copper ions.

Abstract: The present disclosure belongs to the technical field of medicine preparation, and particularly relates to a nasal spray for preventing influenza based on a red pepper extract and heparin and a preparation method thereof. A nasal spray consists of the following raw materials in parts by weight: 10% ?-PGA-CS, 5-10% glycerol, 2% red pepper extract, 5-10% heparin solution, 2-2.4% ethanol and balance of purified water. The present disclosure can not only inhibit germs and diminish inflammation, and has a better therapeutic effect on rhinitis, but also can keep the inner surface of a nasal cavity moist for a long time. Moreover, the present disclosure can reduce side effects on a human body by changing the type of bactericides in the nasal spray, thus keeping the nasal cavity clean and moist for a long time.

Abstract: The present disclosure provides a method for reversibly protecting and separation DNA, comprising phosphorylating the 5?-terminal of a target DNA molecule, modifying the 5?-terminal by adenylation; adding adenosine DNA-sensitive exonuclease to samples obtained after termination of the reaction to digest the template; finally, the obtained adenylated modified DNA, that is, the obtained target DNA is separated, and subjected to technical analysis such as sequencing and identification, and the 5?end of the obtained sequence is the site of adenylation modification. The method provided by the present disclosure fills the gap that the prior art cannot accurately locate the break site on genomic DNA, can realize the quantitative and positioning analysis of the break site on DNA samples of different lengths and different sources, and is simple to use and easy to operate. And there are no special requirements for samples, high accuracy, low detection background influence, and high resolution.