Abstract: The present invention relates generally to conjugates of human ribonucleases and water-soluble polymers, compositions comprising the conjugates and methods of using the same. In particular, the present invention provides conjugates of human ribonucleases and one or more water-soluble polymer compositions (e.g., to increase serum half-life and a pharmacokinetic profile, in vivo biological activity, stability, and/or reduce host immune response to the protein in vivo) as well as methods of using the conjugates in the therapy, treatment, and/or prevention of disease (e.g., cancer).

Abstract: The present invention provides methods and compositions for the isolation and use of membrane proteins and other membrane associated molecules (e.g., peptides, carbohydrates, lipids, or combinations thereof). In particular, the present invention provides amphiphilic polymer compositions and methods of using the compositions to solubilize, enrich and isolate components of biological membranes, including membrane proteins, while retaining function and/or activity of the component.

Abstract: The present invention is directed toward the manufacturing of three-dimensional polymeric coatings for molecule (e.g., protein) immobilization on a solid surface (e.g., surface of a glass slide, microwell plate, etc.). Such surfaces find use as microarrays. In some embodiments, the 3D coating are hydrogel-based and comprise a blend of at least two polymers with distinctive roles: 1) inert 3D support; and 2) protein reactive polymer (e.g., primary amine-reactive polymer) which is able to bond to the glass surface and covalently react with proteins. In some embodiments, the 3D coating possesses superabsorbent properties and comprises a crosslinked polyacrylic acid with carboxylic groups activated to react with primary amines of molecules to be arrayed.

Abstract: The present invention is directed toward the delivery of a toxic protein to pathogenic cells, particularly cancer cells. In preferred embodiments, the toxic protein is a ribonuclease that has been modified to make it toxic to target cells and that can be conjugated to a target cell-specific delivery vector, such as an antibody, for delivery to pathogenic cells.