Abstract: A system and method for sequencing of items in a vertical stack is disclosed. The RFID based sequencing system includes first and second towers positioned about a portal, each of the first and the second towers having at least two strategically, vertically spaced antennas configured to acquire information from RFID tags attached to individual assets in a vertical stack of assets. The system further includes an RFID reader in communication with the antenna and with an asset management system that stores computer executable instructions configured to determine a vertical position of each RFID tag in the vertical stack of assets as it moves through the portal between the first and second towers.