Abstract: A method for vitrification of a tissue or organ includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than ?15° C. to a cryoprotectant concentration sufficient for vitrification; cooling the tissue or organ at an average rate of from 2.5-100° C. per minute to a temperature between ?80° C. and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30° C. per minute to a temperature below the glass transition temperature to vitrify the tissue or organ. After the vitrified tissue or organ has been stored, the tissue or organ may be removed from vitrification by warming the tissue or organ at an average rate of from 20-40° C. per minute to a temperature between ?80° C. and the glass transition temperature; further warming the tissue or organ at a rate greater than 80° C. per minute to a temperature above ?75° C.; and reducing the concentration of the cryoprotectant.

Abstract: An apparatus for adjusting the body temperature of a patient comprises an enclosure defining an interior space for receiving at least a portion of a patient's body therein. The enclosure is adapted for substantially sealingly enclosing the portion of the patient's body within the interior space with the enclosure. Heat transfer liquid may then be circulated through the interior space of the enclosure via an inlet and an outlet for flow over the patient's body in direct liquid contact therewith to promote heat transfer between the patient's body and said heat transfer liquid. The heat transfer liquid may be either warmer or cooler than the patient's body temperature, to either warm or cool the portion. Controlled cooling may be employed to induce therapeutic hypothermia, while controlled warming may be employed to counteract unintended hypothermia.

Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the organ cassette with one or more openings configured to allow tubing to pass through the openings and be connected to the organ or tissue within the cassette, and including a pressure control device to allow pressure inside the portable housing to be varied.

Abstract: An apparatus for adjusting the body temperature of a patient comprises an enclosure defining an interior space for receiving at least a portion of a patient's body therein. The enclosure is adapted for substantially sealingly enclosing the portion of the patient's body within the interior space with the enclosure. Heat transfer liquid may then be circulated through the interior space of the enclosure via an inlet and an outlet for flow over the patient's body in direct liquid contact therewith to promote heat transfer between the patient's body and said heat transfer liquid. The heat transfer liquid may be either warmer or cooler than the patient's body temperature, to either warm or cool the portion. Controlled cooling may be employed to induce therapeutic hypothermia, while controlled warming may be employed to counteract unintended hypothermia.

Abstract: The invention is targeted at the process of separating gas, such as air, from a liquid path. Specifically, the invention provides a means to remove gas from a dynamic liquid path, manage the removed gas and liquid path. The invention provides a means to remove gas from a dynamic liquid path using the buoyant property of gas in a less buoyant liquid, having ingress and egress ports for liquid and gas flow, and separate points of egress for liquid and trapped gas and integral liquid channels.

Abstract: A dispersal pattern of hydrogen bonding sites on an ice surface is used as a template in a process for the design, selection and manufacture of synthetic ice interface dopants. Ice interface dopants are generally molecules which bind to a surface of an ice crystal and inhibit subsequent gain or loss of water molecules. The ice interface dopants can thus inhibit ice crystal growth, recrystallization, and sublimation. Ice interface dopants can also inhibit heterogeneous nucleating agents, and thus postpone or prevent ice nucleation. On the other hand, very strong IIDs may be used as well to beneficially induce ice nucleation. Exemplary dopant structures are provided that achieve near-perfect ice-bonding efficiency while being thoroughly adaptable to a wide variety of specialized ice-bonding applications. Orbital steering provides for steering lone pair orbitals of ice bonding atoms in the interface dopant to result in an optimal angular alignment with the complementary binding sites on ice.

Abstract: A method for vitrification of a tissue or organ includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than −15° C. to a cryoprotectant concentration sufficient for vitrification; cooling the tissue or organ at an average rate of from 2.5-100° C. per minute to a temperature between −80° C. and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30° C. per minute to a temperature below the glass transition temperature to vitrify the tissue or organ. After the vitrified tissue or organ has been stored, the tissue or organ may be removed from vitrification by warming the tissue or organ at an average rate of from 20-40° C. per minute to a temperature between −80° C. and the glass transition temperature; further warming the tissue or organ at a rate of from 200-300° C. per minute to a temperature above −75° C.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.

Abstract: A unified solution system for organ and/or tissue preservation and/or hypothermic blood substitution can be used to prepare multiple solutions for use in various stages of organ procurement, preservation and transplantation and bloodless surgery procedures.

Abstract: A parts separator is provided for separating undesired heavy material from wet chips. The parts separator is adapted to be attached to a centrifugal separator, and includes a first chute member having a top portion and a bottom portion and including spaced open first and second chute ends. The first end is adapted to receive wet chips to be centrifuged and the second end is adapted to be connected to a centrifugal parts separator. The parts separator also includes a heavy material drop out opening disposed in the first chute member bottom portion. The first chute member top has at least one opening disposed therein. The parts separator also includes a second chute member having spaced open first and second ends. The first end of the second chute member is adapted to receive wet chips to be separated and the second chute member end is attached to the first chute member at the location of the opening in the first chute top portion.

Abstract: A unified solution system for organ and/or tissue preservation and/or hypothermic blood substitution can be used to prepare multiple solutions for use in various stages of organ procurement, preservation and transplantation and bloodless surgery procedures.

Abstract: A system for transporting wet chips to a centrifugal separator where the wet chips are separated into dry chips and fluid with the dry chips being transported to a dry chips site and the fluid recirculated or collected. Preferably, the centrifugal separator, when actuated, serves to generate a pneumatic flow of air through a wet chip conduit whereby the wet chips are pneumatically conveyed to the separator from a source of origin such as a tooling machine station where wet chips are generated. A parts separator may be located at either end of the wet chip conduit.

Abstract: The invention teaches methods and compositions for removing thrombi lodged in the microvasculature of an organ. To remove the thrombi, the organ may be perfused, flushed or washed with a suitable perfusion solution to which a sufficient amount of a thrombolytic agent, such as Streptokinase, has been added. The perfusing, flushing or washing process of the organ with the thrombolytic agent will promote thrombolysis on existing thrombi, prevent the formation of new thrombi in the organ, and/or open the vasculature of the organ thereby decreasing vascular resistance and increasing flow. The method of the invention may be practiced using an organ perfusion apparatus that would allow the viability of the organ to be sustained and/or restored upon perfusion with a thrombolytic agent.

Abstract: A system for transporting wet chips to a centrifugal separator where the wet chips are separated into dry chips and fluid with the dry chips being transported to a dry chips site and the fluid recirculated or collected. Preferably, the centrifugal separator, when actuated, serves to generate a pneumatic flow of air through a wet chip conduit whereby the wet chips are pneumatically conveyed to the separator from a source of origin such as a tooling machine station where wet chips are generated. A parts separator may be located at either end of the wet chip conduit.

Abstract: A dispersal pattern of hydrogen bonding sites on an ice surface is used as a template in a process for the design, selection and manufacture of synthetic ice interface dopants. Ice interface dopants are molecules which when bound to a surface of an ice crystal inhibit the incorporation of additional water molecules into the crystal. The ice interface dopants thus inhibit ice crystal growth, recrystallization, and sublimation. Ice interface dopants can also inhibit heterogenous nucleating agents, and thus postpone or prevent ice nucleation. Exemplary dopant structures are provided that achieve near-perfect ice-bonding efficiency while being thoroughly adaptable to a wide variety of specialized ice-bonding applications. Orbital steering provides for steering lone pair orbitals of ice bonding atoms in the interface dopant to result in an optimal angular alignment with the complementary binding sites on ice.

Abstract: A composition including polyethylene glycol (PEG) and glutathione (GSH), is used to treat vascular grafts prior to cryopreservation. The PEG and GSH containing composition is use to treat vascular tissue grafts prior to their cryopreservation to ameliorate the onset of intimal hyperplasia. The composition can also be used to treat vascular tissue grafts prior to cryopreservation by incorporation into solutions used for vascular tissue graft transport and/or in other vascular tissue graft processing steps.