Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
Type:
Application
Filed:
August 22, 2019
Publication date:
July 23, 2020
Applicant:
REG Life Sciences, LLC
Inventors:
Jason J. LUTES, Stephen B. Del Cardayre
Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.
Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
Type:
Grant
Filed:
June 15, 2017
Date of Patent:
August 27, 2019
Assignee:
REG Life Sciences, LLC
Inventors:
Jason J. Lutes, Stephen B. Del Cardayre
Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.
Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.
Type:
Grant
Filed:
September 5, 2017
Date of Patent:
May 28, 2019
Assignee:
Reg Life Sciences, LLC
Inventors:
Derek L. Greenfield, Elizabeth J. Clarke, Eli S. Groban, Vikranth Arlagadda, Sungwon Lee, Xuezhi Li, Zhihao Hu, Baolong Zhu
Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Type:
Grant
Filed:
July 28, 2016
Date of Patent:
February 19, 2019
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Mathew Rude, Na Trinh, Andreas Schirmer, Jacob Gano
Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.
Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
Type:
Application
Filed:
December 13, 2016
Publication date:
January 3, 2019
Applicant:
REG Life Sciences, LLC
Inventors:
Andreas Schirmer, Baolong Zhu, Emanuela Popova
Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.
Type:
Grant
Filed:
October 4, 2016
Date of Patent:
December 11, 2018
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Andreas W. Schirmer, Mathew A. Rude, Shane A. Brubaker
Abstract: Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use.
Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.
Type:
Grant
Filed:
February 22, 2017
Date of Patent:
April 24, 2018
Assignee:
REG Life Sciences, LLC
Inventors:
Louis Hom, Na Trinh, Murtaza Alibhai, Zhihao Hu, Eli Groban, Vikranth Arlagadda, Elizabeth Clarke
Abstract: The disclosure relates to the field of specialty chemicals. In particular, the disclosure provides novel 1,3-fatty-diol compounds and derivatives thereof which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc.
Abstract: The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
January 30, 2018
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Andrew M. Shumaker, Bernardo M. Da Costa, Kevin Holden, Louis G. Hom, Tarah S. Baron, Noah Helman
Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Type:
Grant
Filed:
June 13, 2017
Date of Patent:
January 23, 2018
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Mathew Rude, Na Trinh, Andreas Schirmer, Jacob Gano
Abstract: The disclosure relates to a novel glycerol purification process that produces partially refined waste glycerol for a variety of industrial applications. The disclosure encompasses a salt-containing partially refined glycerol composition that is suitable as a fermentation grade glycerol.
Type:
Grant
Filed:
August 19, 2014
Date of Patent:
January 16, 2018
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Myong K. Ko, Fernando A. Sanchez-Riera, Simon Li, Perry Y. Liao
Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.
Abstract: Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use.
Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.
Type:
Grant
Filed:
May 13, 2016
Date of Patent:
September 12, 2017
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Derek L. Greenfield, Elizabeth Clarke, Eli Groban, Vikranth Arlagadda, Sungwon Lee, Xuezhi Li, Zhihao Hu, Baolong Zhu
Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Type:
Grant
Filed:
January 16, 2014
Date of Patent:
June 20, 2017
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Mathew Rude, Na Trinh, Andreas Schirmer, Jacob Gano