Abstract: The disclosure relates to the biosynthesis of cannabinoids and related prenylated phenolic compounds using recombinant enzymes. In particular, the disclosure provides recombinant mutant ORF2 enzymes engineered to produce a greater amount of a desired product, or to have a greater ability to catalyze a reaction using a desired substrate, as compared to WT ORF2. The disclosure also provides methods of preparing such ORF2 mutant enzymes; as well as methods of use thereof in improving the biosynthesis of cannabinoids and related prenylated phenolic compounds.
Type:
Grant
Filed:
November 13, 2019
Date of Patent:
January 19, 2021
Assignee:
RENEW BIOPHARMA, INC.
Inventors:
Michael Mendez, Joseph Noel, Michael Burkart, Jeremy Lanoiselee, Kyle Botsch, Matthew Saunders