Patents Assigned to Rensselaer Polytechin Institute
  • High affinity, low molecular weight displacers for oligonucleotide purification
    Patent number: 6573373
    Abstract: A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
    Type: Grant
    Filed: June 29, 1999
    Date of Patent: June 3, 2003
    Assignees: ISIS Pharmaceuticals, Inc., Rensselaer Polytechin Institute
    Inventors: Abhinav A. Shukla, Ranjit R. Deshmukh, Steven M. Cramer, James A. Moore