Patents Assigned to Repligen Corporation
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Patent number: 12275774Abstract: The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.Type: GrantFiled: December 7, 2022Date of Patent: April 15, 2025Assignee: Repligen CorporationInventors: Erik Fiedler, Ulrich Haupts
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Publication number: 20250116349Abstract: The disclosure is directed to pneumatic actuator assemblies for reduced dead leg. In one approach, an assembly may include a valve body, and a first actuator and a second actuator each coupled to the valve body by a bonnet assembly. The bonnet assembly may include a bonnet body having a first end opposite a second end, wherein the first end is connected with the valve body, and a nut threadedly connected to the second end of the bonnet body. The bonnet assembly may further include an actuator bonnet, wherein the nut partially surrounds the actuator bonnet, and wherein the actuator bonnet is rotatable about a lengthwise axis extending through a center of a stem of a piston.Type: ApplicationFiled: September 30, 2024Publication date: April 10, 2025Applicant: Repligen CorporationInventors: Anthony Mauricio Landi Adib, Patrick Lyng
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Patent number: 12257530Abstract: The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.Type: GrantFiled: September 5, 2023Date of Patent: March 25, 2025Assignee: Repligen CorporationInventors: Erik Fiedler, Ulrich Haupts
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Patent number: 12253189Abstract: An encapsulated valve system includes a first housing portion having a first facing surface, the first facing surface comprising a plurality of branch pathways formed as a recess within the first facing surface. The valve system further includes a second housing portion having a second facing surface, the second facing surface comprising a plurality of branch pathways formed as a recess within the second facing surface. A disposable conduit is configured to be interposed between the first and second housing portions and disposed within the recess of the first facing surface and the recess of the second facing surface. The disposable conduit is thus sandwiched between the first and second facing surfaces. A plurality of pinch valve actuators are mounted on one or both of the first housing portion and the second housing portion, the plurality of pinch valve actuators configured to pinch the disposable conduit at selective branch pathways.Type: GrantFiled: March 9, 2022Date of Patent: March 18, 2025Assignee: Repligen CorporationInventors: Michael C. Gagne, Steven V. Cates
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Publication number: 20250051400Abstract: The present invention relates to Fc binding proteins comprising one or more Fc binding domains wherein at least one domain comprises of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-6 or 21. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.Type: ApplicationFiled: September 3, 2024Publication date: February 13, 2025Applicant: Repligen CorporationInventors: Paul Knick, Erik Fiedler, Ulrich Haupts, Maren Meysing
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Publication number: 20250032997Abstract: The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.Type: ApplicationFiled: July 23, 2024Publication date: January 30, 2025Applicant: Repligen CorporationInventors: Michael Bransby, Philip Yuen, Derek Carroll
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Publication number: 20250027032Abstract: The invention provides tangential flow filtration systems and methods for capture and harvest of a biologic product, and related compositions.Type: ApplicationFiled: July 17, 2024Publication date: January 23, 2025Applicant: Repligen CorporationInventors: Michael Bransby, Philip Yuen, Westley Blacklock, Marlon Tannous, Karol Lacki, Anthony Hunt, James Peyser
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Patent number: 12181076Abstract: An encapsulated valve system includes a first housing portion having a first facing surface, the first facing surface comprising a plurality of branch pathways formed as a recess within the first facing surface. The valve system further includes a second housing portion having a second facing surface, the second facing surface comprising a plurality of branch pathways formed as a recess within the second facing surface. A disposable conduit is configured to be interposed between the first and second housing portions and disposed within the recess of the first facing surface and the recess of the second facing surface. The disposable conduit is thus sandwiched between the first and second facing surfaces. A plurality of pinch valve actuators are mounted on one or both of the first housing portion and the second housing portion, the plurality of pinch valve actuators configured to pinch the disposable conduit at selective branch pathways.Type: GrantFiled: May 3, 2021Date of Patent: December 31, 2024Assignee: Repligen CorporationInventors: Michael C. Gagne, Steven V. Cates
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Publication number: 20240335776Abstract: A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.Type: ApplicationFiled: June 20, 2024Publication date: October 10, 2024Applicant: Repligen CorporationInventors: Rudolf Pavlik, Ralf Kuriyel
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Publication number: 20240141891Abstract: The present disclosure relates to diaphragm pumps with one or more filters, e.g., tangential flow filters, membranes, and ultrafiltration membranes, for various applications, including, e.g., bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same. In an aspect, a pump for alternating tangential flow filtration may include a diaphragm. A housing may contain the diaphragm. The housing may have a first end open to a retentate channel of a first membrane. A magnetic member may be coupled to the diaphragm. An electromagnetic field generator may be disposed about the magnetic member.Type: ApplicationFiled: January 11, 2022Publication date: May 2, 2024Applicant: Repligen CorporationInventor: Rudolf Pavlik
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Publication number: 20240133472Abstract: A valve assembly with a multidirectional flow path includes a valve body that includes respective hinged portions defining respective passageways extending through the valve body when in a closed state. A flexible conduit or tubing having a loop portion and a plurality of branches extending from the loop portion is at least partially positioned within the valve body. Portions of the loop that extend beyond the valve body are encapsulated by respective rigid jackets. Multiple valves are situated on the valve body and are used to selectively pinch the loop portion in different pinching configurations to direct fluid to or from the particular branches. The valve assembly may be integrated into a manufacturing or other production process with optional additional jackets used to encapsulate the flexible conduit branches.Type: ApplicationFiled: January 5, 2024Publication date: April 25, 2024Applicant: Repligen CorporationInventors: Michael C. Gagne, Steven V. Cates, Dean C. Richards, Scott Bendon
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Publication number: 20240100450Abstract: If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.Type: ApplicationFiled: February 6, 2018Publication date: March 28, 2024Applicant: Repligen CorporationInventors: James Ronald Peyser, Dana Cristina Pentia
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Publication number: 20240091671Abstract: The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.Type: ApplicationFiled: September 5, 2023Publication date: March 21, 2024Applicant: Repligen CorporationInventors: Erik Fiedler, Ulrich Haupts
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Patent number: 11933686Abstract: A pressure gauge and/or relief valve which may be used in a single-use fluid system, such as a sterilizable fluid system. The pressure gauge and/or pressure relief valve may be configured to measure and to relieve fluid within a fluid-containing structure, the pressure gauge relief valve including a housing having a first end, a second end, and an inner surface defining a passage between the first end and the second end, a movable element movable within the passage, wherein the housing first end is configured to be coupled with a fluid-containing structure, the housing second end is spaced from the housing first end; and the movable element is movable with respect to the housing to indicate pressure therein and to allow fluid to pass between the movable element and the inner surface of the passage at a selected pressure.Type: GrantFiled: June 29, 2022Date of Patent: March 19, 2024Assignee: Repligen CorporationInventor: Travis R. Ward
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Patent number: 11865478Abstract: A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.Type: GrantFiled: August 2, 2022Date of Patent: January 9, 2024Assignee: Repligen CorporationInventor: Rudolf Pavlik
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Publication number: 20230400405Abstract: A compact light source for coupling to a movable optic probe may include a light emitting diode (LED), to generate a probe signal at a first bandwidth, an optic coupler, disposed adjacent to the LED, and arranged to couple the probe signal into the movable probe, and a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth.Type: ApplicationFiled: May 17, 2023Publication date: December 14, 2023Applicant: Repligen CorporationInventors: Matthew Muller, Richard Hall, III, Yusheng Zhang, Peter Halatin
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Patent number: 11834646Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.Type: GrantFiled: May 24, 2022Date of Patent: December 5, 2023Assignee: Repligen CorporationInventor: Rudolf Pavlik
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Publication number: 20230383241Abstract: A bleed recovery system for use in a bioreactor system is disclosed. The bleed recovery system being configured to recover product of interest from the bleed material of the bioreactor system. In one embodiment, the bioreactor system includes a bleed recovery system for removing cell cultures from the bioreactor. The bleed recovery system includes a bleed pump coupled to the bioreactor so that cell bleed material can be removed from the bioreactor, a bleed vessel for receiving the cell bleed material, a cell retention device coupled to the bleed vessel, a harvest pump coupled to the cell retention device to deposit the product of interest into a second harvest tank, and a bleed pump connected to the bleed vessel to deposit the spent bleed material into a waste tank. Thus arranged, some or all of the product of interest can be separated from the spent medium.Type: ApplicationFiled: May 22, 2023Publication date: November 30, 2023Applicant: Repligen CorporationInventors: Charles Hill, Joey Tse, Mario Sinani, Earl Pineda
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Publication number: 20230375468Abstract: An apparatus may include a light emitting diode (LED) assembly, comprising a plurality of LEDs that output radiation at a plurality of different wavelengths, respectively. The LED assembly may be arranged to output a composite probe signal at a plurality of instances, wherein the composite probe signal comprises a plurality of probe signals, generated from the plurality of LEDs, respectively. The apparatus may also include a measurement module that includes an optic probe to direct the composite probe signal through a fluid sample, while moving between a plurality of probe positions at the plurality of instances. The measurement module may include a detector, disposed to detect, at the plurality of instances, a transmitted intensity of the composite probe signal at the plurality of different wavelengths after the composite probe signal passes through the fluid sample.Type: ApplicationFiled: May 17, 2023Publication date: November 23, 2023Applicant: Repligen CorporationInventors: Matthew Muller, Richard Hall, III, Yusheng Zhang, Peter Halatin
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Publication number: 20230375467Abstract: A system may include a light source, to generate a probe signal, comprising incident radiation; an optical probe, to direct the incident radiation through a fluid sample; a motor, to move the optical probe along a probe axis, to change a path length of the incident radiation through the fluid sample; and a detector, to receive the incident radiation as attenuated radiation after passing through the fluid sample. The system may include a control system, arranged to: initiate an absorbance measurement by directing movement of the optical probe along the probe axis; direct the light source to emit the incident radiation; and automatically adjust at least one measurement parameter of a set of measurement parameters for the sample measurement, based upon a slope parameter m, wherein m is derived from a rate of change in an intensity of the attenuated radiation with a change in the path length.Type: ApplicationFiled: May 17, 2023Publication date: November 23, 2023Applicant: Repligen CorporationInventors: Matthew Muller, Richard Hall, III, Yusheng Zhang, Peter Halatin