Patents Assigned to Repligen Corporation
  • Use of thioredoxin, thioredoxin-derived, or thioredoxin-like dithiol peptides in hair care preparations
    Patent number: 5028419
    Abstract: The subject invention enables a more efficient management of hair by providing a novel preparation for waving, straightening, softening, or removing hair, employing as a key ingredient the compound thioredoxin or a thioredoxin-derived, or thioredoxin-like, dithiol peptide in combination with a sulfite or bisulfite compound.
    Type: Grant
    Filed: August 23, 1989
    Date of Patent: July 2, 1991
    Assignee: Repligen Corporation
    Inventor: Vincent P. Pigiet
  • Novel pyrroloquinoline alkaloids and methods of use
    Patent number: 5028613
    Abstract: The subject invention pertains to novel compounds, batzellines and isobatzellines, isolated from marine sponges. These compounds have been shown to possess antitumor activity. Various derivatives of these compounds have been prepared. The procedures for the isolation of the parent compounds and the preparation of some of their derivatives are described.
    Type: Grant
    Filed: February 16, 1990
    Date of Patent: July 2, 1991
    Assignee: Repligen Corporation
    Inventors: H. Howard Sun, Shin-ishi Sakemi
  • Efficient prokaryotic expression system
    Patent number: 4952682
    Abstract: A novel highly effective prokaryotic expression system is exemplified specifically by being used to produce the useful enzyme .beta.-glucuronidase (BG). This system uses a hybrid plasmid comprising BG gene promoter DNA. The level of expression of BG by an E. coli K-12 derivative host is in the 50% of total cellular protein range. The invention expression system also can be used to express other useful proteins, as disclosed herein.
    Type: Grant
    Filed: September 30, 1988
    Date of Patent: August 28, 1990
    Assignee: Repligen Corporation
    Inventors: Algis Anilionis, John L. Palmer
  • System for release of proteins from microbe cells
    Patent number: 4948735
    Abstract: The invention concerns novel and useful mutant microbes which are capable of releasing substantial amounts of any of several periplasmic or recombinant proteins into the culture medium when carrying an expressed Kil gene. Though E. coli are exemplified, the invention is broadly applicable to the making of mutants of other microbes, for example, Salmonella, Klebsiella, and Rhizobium. A key feature of the invention is the use of a novel selection procedure employing a plasmid comprising the kil gene.
    Type: Grant
    Filed: February 6, 1987
    Date of Patent: August 14, 1990
    Assignee: Repligen Corporation
    Inventors: Salvador E. Luria, Joan L. Suit, Jennifer A. Jackson
  • Hair removing composition and method containing thioredoxin, thioredoxin-derived, or thioredoxin-like dithiol peptides
    Patent number: 4941885
    Abstract: The subject invention enables a more efficient management of hair by providing a novel preparation for waving, straightening, softening, or removing hair, employing as a key ingredient the compound thioredoxin or a thioredoxin-derived, or thioredoxin-like, dithiol peptide in combination with thioglycolic acid compounds. This invention allows hair to be treated at a lower pH to minimize hair damage when waving, straightening, or softening the hair; when used to remove hair, objectionable odors of commercial depilatories are minimized or eliminated.
    Type: Grant
    Filed: September 14, 1989
    Date of Patent: July 17, 1990
    Assignee: Repligen Corporation
    Inventor: Vincent P. Pigiet
  • Use of thioredoxin, thioredoxin-derived, or thioredoxin-like dithiol peptides in hair care preparations
    Patent number: 4935231
    Abstract: The subject invention enables a more efficient management of hair by providing a novel preparation for waving, straightening, softening, or removing hair, employing as a key ingredient the compound thioredoxin or a thioredoxin-derived, or thioredoxin-like, dithiol peptide in combination with an organic reducing agent. This invention allows hair to be treated at a lower pH to minimize hair damage when waving, straightening, or softening the hair; when used to remove hair, objectionable odors of commercial depilatories are minimized or eliminated.
    Type: Grant
    Filed: January 4, 1988
    Date of Patent: June 19, 1990
    Assignee: Repligen Corporation
    Inventor: Vincent P. Pigiet
  • Use of thioredoxin, thioredoxin-derived, or thioredoxin-like dithiol peptides in hair care preparations
    Patent number: 4919924
    Abstract: The subject invention enables a more efficient management of hair by providing a novel preparation for waving, straightening, softening, or removing hair, employing as a key ingredient the compound thioredoxin or a thioredoxin-derived, or thioredoxin-like, dithiol peptide in combination with thioglycolic acid compounds. This invention allows hair to be treated at a lower pH to minimize hair damage when waving, straightening, or softening the hair; when used to remove hair, objectionable odors of commercial depilatories are minimized or eliminated.
    Type: Grant
    Filed: August 28, 1985
    Date of Patent: April 24, 1990
    Assignee: Repligen Corporation
    Inventor: Vincent P. Pigiet
  • Thioredoxin shufflease and use thereof
    Patent number: 4904602
    Abstract: The subject invention concerns a novel enzyme named thioredoxin shufflease, means for preparing the same, and procedures for using thioredoxin shufflease to fold proteins containing disulfide crosslinks. Thioredoxin shufflease is a generic term to define enzymes which have the following characteristics: (a) contain a single reactive thiol group; (b) catalyze the exchange of disulfides in a protein undergoing the refolding process; and (c) are not consumed in the oxidation/refolding process. Specifically exemplified is a thioredoxin shufflease produced from an E. coli thioredoxin gene.
    Type: Grant
    Filed: August 8, 1986
    Date of Patent: February 27, 1990
    Assignee: Repligen Corporation
    Inventors: Vincent P. Pigiet, James R. Rusche, Barbara J. Schuster
  • Use of thioredoxin, thioredoxin-derived, or thioredoxin-like dithiol peptides in hair care preparations
    Patent number: 4894223
    Abstract: The subject invention enables a more efficient management of hair by providing a novel preparation for waving, straightening, softening, or removing hair, employing as a key ingredient the compound thioredoxin or a thioredoxin-derived, or thioredoxin-like, dithiol peptide in combination with a sulfite or bisulfite compound.
    Type: Grant
    Filed: January 4, 1988
    Date of Patent: January 16, 1990
    Assignee: Repligen Corporation
    Inventor: Vincent P. Pigiet
  • Hybrid proteins produced by an ultrahigh prokaryotic expression
    Patent number: 4888280
    Abstract: Hybrid useful proteins are prepared by a novel biological system comprising a prokaryotic host transformed with novel hybrid plasmids' .beta.-glucuronidase (BG) gene DNZ and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2.DELTA.n express >60% of the desired fusion protein having protein A-like biological activity. Other useful proteins can be expressed via the elegant highly efficient expression system of the subject invention.
    Type: Grant
    Filed: August 25, 1986
    Date of Patent: December 19, 1989
    Assignee: Repligen Corporation
    Inventors: John L. Palmer, Algis Anilionis
  • Dinucleoside pyrophosphates and pyrophosphate homologs as plant antivirals
    Patent number: 4855304
    Abstract: Potent plant antivirals are disclosed along with their method of use. These antivirals, 5',5'- or 2',5'- or 3',5'-pyrophosphorylated-dinucleotides, are specifically shown to inhibit tobacco mosaic virus (TMV) replication in tobacco plants when applied to the leaves or through the roots of these plants. These antiviral compounds can also be used to treat a variety of other plants, as disclosed herein, which are susceptible to a broad range of viruses.
    Type: Grant
    Filed: October 10, 1985
    Date of Patent: August 8, 1989
    Assignee: Repligen Corporation
    Inventor: Yair Devash
  • Use of hydroxylated indoles as dye precursors
    Patent number: 4776857
    Abstract: The invention concerns a new system to dye hair and/or skin. The system comprises the use of a hydroxyindole dye precursor and sodium periodate. Advantageously, this dye system allows the dye to penetrate inside the hair fiber without coating the hair shaft and, thus, give a more pleasing and stable dye result.
    Type: Grant
    Filed: November 21, 1986
    Date of Patent: October 11, 1988
    Assignee: Repligen Corporation
    Inventors: James Carroll, Cynthia D. Millis, Walter C. Herlihy
  • Hair dye composition and process for using the same
    Patent number: 4746322
    Abstract: The subject invention concerns novel dye compositions and a process for dyeing keratinous fibers, for example, hair, to give a pleasing and stable colored product. The dye composition is a novel combination of three elements to give a dye that, unexpectedly, has highly desirable dyeing properties. This unique combination comprises (1) a suitable organic compound to assist dye dispersion, (2) dye precursors including dopamine, D-dopa, L-dopa, D,L-dopa, or analogs thereof, and (3) iodate or periodate. Desired color variations can be obtained by the use of appropriate color modifiers.
    Type: Grant
    Filed: May 11, 1987
    Date of Patent: May 24, 1988
    Assignee: Repligen Corporation
    Inventor: Walter C. Herlihy
  • Use of thioredoxin, thioredoxin-derived, or thioredoxin-like dithiol peptides in hair care preparations
    Patent number: 4738841
    Abstract: The subject invention enables a more efficient management of hair by providing a novel preparation for waving, straightening, softening, or removing hair, employing as a key ingredient the compound thioredoxin or a thioredoxin-derived, or thioredoxin-like, dithiol peptide in combination with thioglycolic acid compounds. This invention allows hair to be treated at a lower pH to minimize hair damage when waving, straightening, or softening the hair; when used to remove hair, objectionable odors of commercial depilatories are minimized or eliminated.
    Type: Grant
    Filed: August 25, 1986
    Date of Patent: April 19, 1988
    Assignee: Repligen Corporation
    Inventor: Vincent P. Pigiet
  • Efficient prokaryotic expression system using portions of the E. coli .beta .
    Patent number: 4721671
    Abstract: A novel highly effective prokaryotic expression system is exemplified specifically by being used to produce the useful enzyme .beta.-glucuronidase (BG). This system uses a hybrid plasmid comprising BG gene promotor DNA. The level of expression of BG by an E. coli K-12 derivative host is in the 50% of total cellular protein range. The invention expression system also can be used to express other useful proteins, as disclosed herein.
    Type: Grant
    Filed: December 26, 1984
    Date of Patent: January 26, 1988
    Assignee: Repligen Corporation
    Inventors: Algis Anilionis, John L. Palmer
  • Use of rLDM.TM. 1-6 and other ligninolytic enzymes for the decolorization of E1 effluent
    Patent number: 4692413
    Abstract: Disclosed is a novel process for decolorization of E1 effluent. Specifically, novel enzymes, designated rLDM.TM., and other ligninolytic enzymes present in the extracellular growth medium from a fermentation of Phanerochaete chrysosporium, are used to decolorize the effluent.
    Type: Grant
    Filed: March 28, 1986
    Date of Patent: September 8, 1987
    Assignee: Repligen Corporation
    Inventor: Roberta L. Farrell
  • Hybrid proteins produced by an ultrahigh prokaryotic expression system
    Patent number: 4691009
    Abstract: Hybrid useful proteins are prepared by a novel biological system comprising a prokaryotic host transformed with novel hybrid plasmids' .beta.-glucuronidase (BG) gene DNA and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2.DELTA.N express >60% of the desired fusion protein having protein A-like biological activity. Other useful proteins can be expressed via the elegant highly efficient expression system of the subject invention.
    Type: Grant
    Filed: December 26, 1984
    Date of Patent: September 1, 1987
    Assignee: Repligen Corporation
    Inventors: John L. Palmer, Algis Anilionis
  • Use of rLDM.TM. 1-6 and other ligninolytic enzymes in the bleaching of kraft pulp
    Patent number: 4690895
    Abstract: The subject invention concerns a novel enzymatic process for bleaching kraft pulp. Specifically, novel enzymes, designated rLDM.TM., and other ligninolytic enzymes present in the extracellular growth medium from a fermentation of Phanerochaete chrysosporium, are used to bleach kraft pulp to a desired lighter color.
    Type: Grant
    Filed: March 28, 1986
    Date of Patent: September 1, 1987
    Assignee: Repligen Corporation
    Inventor: Roberta L. Farrell
  • Use of rLDM.TM. 1-6 and other ligninolytic enzymes in the treatment of mechanical pulps
    Patent number: 4687745
    Abstract: Disclosed is a novel process for enhancing the strength properties and brightness stability of mechanical pulps. The process uses novel enzymes called rLDM.TM. and other ligninolytic enzymes present in the extracellular growth medium of a fermentation of Phanerochaete chrysosporium.
    Type: Grant
    Filed: March 28, 1986
    Date of Patent: August 18, 1987
    Assignee: Repligen Corporation
    Inventor: Roberta L. Farrell
  • Novel enzymes which catalyze the degradation and modification of lignin
    Patent number: 4687741
    Abstract: Novel lignin-degrading enzymes designated rLDM.TM.1, rLDM.TM.2, rLDM.TM.3, rLDM.TM.4, rLDM.TM.5, and rLDM.TM.6 are isolated and purified to the essentially pure form, wherein each rLDM.TM. is substantially free of other rLDM.TM. and native proteins, from the extracellular medium of a novel mutant microbe. The novel mutant, designated SC26, produces large amounts of the rLDM.TM., thus facilitating the isolation and purification of them. These rLDM.TM. are useful in pulping processes to degrade and/or modify lignin.
    Type: Grant
    Filed: March 28, 1986
    Date of Patent: August 18, 1987
    Assignees: Repligen Corporation, The United States of America as represented by the Secretary of Agriculture
    Inventors: Roberta L. Farrell, Thomas K. Kirk, Ming Tien