Abstract: Disclosed is a method for producing a DHA phospholipid comprising an ?3 unsaturated fatty acid, particularly DHA, as a constituent lipid by using a microorganism in a simpler manner. Specifically disclosed is a method for producing a phospholipid comprising an ?3 unsaturated fatty acid as a constituent lipid, which comprises the steps of: growing a microorganism capable of producing the ?3 unsaturated fatty acid in a culture medium containing a carbon source; and further culturing the grown microorganism in a culture medium without any carbon source. The method enables to produce a highly value-added phospholipid which comprises an ?3 unsaturated fatty acid as a constituent lipid by using a microorganism capable of producing the ?3 unsaturated fatty acid in a large quantity.