Abstract: This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.

Abstract: According to an embodiment, a signal processing apparatus includes an estimation unit and an updating unit. The estimation unit is configured to estimate an auxiliary variable of a target section including first and second sections of input signals by using an approximating auxiliary function for approximating an auxiliary function having an auxiliary variable as an argument. The auxiliary function is determined according to an objective function that outputs a function value that is smaller as a statistical independence of separated signals into which input signals in time-series are separated by a demixing matrix is higher. The estimation unit is configured to estimate a value of the auxiliary variable of the target section based on the estimated auxiliary variable. The updating unit is configured to update the demixing matrix such that a function value of the approximating auxiliary function is minimized.

Abstract: For each pair of a plurality of slave lasers B for which injection synchronization is performed by a master laser M, by controlling the intensity of light exchanged between two slave lasers B and an optical path length between the two slave lasers B using a slave-to-laser intensity control unit IA and an inter-slave laser optical path length control unit IP, the magnitude and the sign of pseudo ising interaction Jij between the two slave lasers B are implemented. After the plurality of slave lasers B arrive at a steady state, by measuring relative values of the oscillation phases of the plurality of slave lasers B with respect to the oscillation phase of the master laser M by using an oscillation phase measuring unit PM, pseudo ising spins ?i of the plurality of slave lasers B are measured.

Abstract: An information processing unit and information processing method capable of performing a ground-state search of an Ising model having coefficients of arbitrary values regardless of restrictions on hardware or software is suggested. When a ground state of an original problem, which is an Ising model, or an approximate solution of that ground state is calculated as a solution of the original problem, one or more sub-problems which are Ising models are generated from the original problem and the information processing unit searches the ground state of each generated sub-problem and generates a solution of the original problem based on a solution of each sub-problem obtained from the search; and when types of coefficient values of an Ising model whose ground state can be searched are limited, sub-problems which are Ising models composed of the types of limited values of coefficients are generated.

Abstract: A method is provided of creating an end-to-end entanglement (87) between qubits in first and second end nodes (81, 82) of a chain of optically-coupled nodes whose intermediate nodes (80) are quantum repeaters. Local entanglements (85) are created on an on-going basis between qubits in neighboring pairs in the chain through interaction of the qubits with light fields transmitted between the nodes. The quantum repeaters (80) are cyclically operated with their top-level operating cycles being synchronized. Once every top-level operating cycle, each repeater (80) initiates a merging of two entanglements involving respective repeater qubits that are at least expected to be entangled with qubits in nodes disposed in opposite directions along the chain from the repeater. A quantum repeater (80) adapted for implementing this method is also provided.

Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: The path management controller 1 refers to resource information 131 including reservation statuses of working paths and advance reserved paths that are set in every link, so as to calculate a route for setting a new path and set this path in this calculated route. If the route for setting this new path cannot be obtained (1) because of a shortage of residual bandwidth of a link of interest, the path management controller 1 refers to the resource reservation information 131 of working paths and advance reserved paths and calculates an alternative route for another existing path used in the link of interest, and moves this path to the calculated alternative route.

Abstract: For each pair of a plurality of slave lasers B for which injection synchronization is performed by a master laser M, by controlling the intensity of light exchanged between two slave lasers B and an optical path length between the two slave lasers B using a slave-to-laser intensity control unit IA and an inter-slave laser optical path length control unit IP, the magnitude and the sign of pseudo ising interaction Jij between the two slave lasers B are implemented. After the plurality of slave lasers B arrive at a steady state, by measuring relative values of the oscillation phases of the plurality of slave lasers B with respect to the oscillation phase of the master laser M by using an oscillation phase measuring unit PM, pseudo ising spins ?i of the plurality of slave lasers B are measured.

Abstract: According to an embodiment, a signal processing apparatus includes an estimation unit and an updating unit. The estimation unit is configured to estimate an auxiliary variable of a target section including first and second sections of input signals by using an approximating auxiliary function for approximating an auxiliary function having an auxiliary variable as an argument. The auxiliary function is determined according to an objective function that outputs a function value that is smaller as a statistical independence of separated signals into which input signals in time-series are separated by a demixing matrix is higher. The estimation unit is configured to estimate a value of the auxiliary variable of the target section based on the estimated auxiliary variable. The updating unit is configured to update the demixing matrix such that a function value of the approximating auxiliary function is minimized.

Abstract: A route switching method for switching a route in a core network that accommodates service networks including user node apparatuses by use of a server storing path information that includes, in every time frame, a route for a working path and a route for a backup path to be switched over from the working path, both of the working path and the backup path established between two boundary node apparatuses each of which is located on a boundary between the service network and the core network. The server calculates, in response to the connection request from the user node apparatus, a route for a working path and a route for a backup path, and requests the boundary node apparatus to establish this working path. The server can send the route for the backup path to the boundary node apparatus before a failure occurs in the core network.

Abstract: A cantilever for measuring intra-cellular and inter-cellular microspaces of the present invention includes a support portion, a lever portion provided to the support portion so as to protrude therefrom, and a probe portion provided near a free end of the lever portion. The probe portion includes a conductive probe made of a carbon-based material, and an insulating film to coat a periphery of the conductive probe.

Abstract: A method, featuring robustness against changes in tire size, is provided for determining a road surface condition by dividing a time-series waveform of tire vibrations into windows without resorting to detection of the peak positions or measurement of the wheel speed. A time-series waveform of tire vibrations detected by a tire vibration detecting unit is windowed by a windowing unit. Time-series waveforms are extracted from the respective time windows, feature vectors X are calculated therefor, and then likelihoods Z for road-surface HMMs (hidden Markov models) are calculated. The likelihoods Z1 to Z5 calculated for the respective road-surface HMMs are compared with one another, and a road surface condition corresponding to the road-surface HMM showing the highest likelihood is determined to be the condition of the road surface on which the tire is running.

Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.

Abstract: A continuous temperature-gradient incubation apparatus designed to solve the problem of moving of liquid vapor generated on the high-temperature side toward the low-temperature side to thereby cause condensation.

Abstract: In order to disclose an emission allowance for each product and allow its trading on a product unit basis, the present invention manages, in a management server requesting execution of a settlement process to settlement servers which make settlement of the emission allowance, weight information of the emission allowance and identification information of a non-contact IC medium in association with each other and stores, in databases provided in the settlement servers, weight information of an emission allowance on a seller's side, seller's account information, weight information of an emission allowance on a buyer's side, and buyer's account information.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: Genetically modified mammalian cells comprising a Tol2 transposon transferred into a chromosome can be obtained by co-transfecting mammalian cells with a Tol2 transposase encoded by a Tol2 transposon found in medaka fish, and a Tol2 transposon lacking this transposase.

Abstract: A cantilever for measuring intra-cellular and inter-cellular microspaces of the present invention includes a support portion, a lever portion provided to the support portion so as to protrude therefrom, and a probe portion provided near a free end of the lever portion. The probe portion includes a conductive probe made of a carbon-based material, and an insulating film to coat a periphery of the conductive probe.

Abstract: A transmitter sets a time length, determines a time point at which phases of frequency signals coincide within a range of the time length, defines a determined time point as a time reference point, aligns the phases of the frequency signals based on the time reference point, combines phase-aligned frequency, and transmits combined frequency signals to a receiver by the time length as the communication signals. The receiver receives the communication signals, extracts the phases of the frequency signals, obtains a time point at which the phases coincide, and determines an obtained time point as the time reference point.