Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: This computation device and method exponentially shortens a computation time of an NP-complete problem or the like mapped into an Ising model by exponentially shortening a computation time of the Ising mode. For each pair of a plurality of slave lasers, by controlling the intensity, the polarization, and the phase of light exchanged between two slave lasers using an attenuator and a wave plate, the magnitude and the sign of pseudo Ising interaction between two slave lasers are implemented. Then, after the plurality of slave lasers arrive at a steady state, by measuring the polarization of light generated by each slave laser with left-handed circular polarization and right-handed circular polarization used as bases, a pseudo spin of each slave laser is measured.

Abstract: A specimen temperature adjusting apparatus includes a specimen stage that the observation specimen is to be placed on and a temperature adjustment element that is attached to the specimen stage. The specimen stage has a groove surrounding a portion where the observation specimen is to be placed. The temperature adjustment element is located in the groove of the specimen stage.