Abstract: The present disclosure relates to a bioprinter printing assembly and a bioprinter, wherein the printing assembly comprises: a support body and a rotary rod provided with a nutrient solution supply port, wherein the rotary rod is rotatably supported on the support body, a surface of the rotary rod is used for receiving bio-ink provided by a printing spray head, the rotary rod includes a hollow tube section having a central through hole, and a tube wall of the hollow tube section is provided with a permeable hole communicating with the central through hole, and the nutrient solution supply port communicates with the central through hole of the rotary rod to guide external nutrient solution into the rotary rod.