Abstract: A residual material withdrawal control method and a bio-printer are provided. The residual material withdrawal control method includes: obtaining a length of a printing material attached to an exterior of a spray head (12) of a printer; and performing multiple withdrawals on the printing material attached to the exterior of the spray head (12) by a material driving mechanism until the length of the printing material attached to the exterior of the spray head (12) is not higher than an allowable value, according to the length of the printing material. The residual material withdrawal control method can effectively eliminate the printing material attached to the spray head (12) of the printer.

Abstract: The present disclosure provides a bioprinter nozzle, a bioprinter and a lumen tissue construct printing method. The bioprinter nozzle includes: a bio-ink flow channel disposed inside the bioprinter nozzle; a bio-ink ejection port in communication with the bio-ink flow channel and located on the lateral side of an ejection end of the bioprinter nozzle; a medical adhesive flow channel disposed inside the bioprinter nozzle and isolated from the bio-ink flow channel; and a medical adhesive ejection port in communication with the medical adhesive flow channel and disposed on the lateral side of the ejection end, the medical adhesive ejection port and the bio-ink ejection port being arranged in a staggered manner in the axial direction of the bioprinter nozzle. The bioprinter includes the bioprinter nozzle. The lumen tissue construct printing method uses the bioprinter for printing.

Abstract: The present disclosure provides a bioprinter spray head assembly and a bioprinter, wherein the spray head assembly is internally provided with a first flow channel and a second flow channel, an outlet of the first flow channel is adjacent to an outlet of the second flow channel, and the outlet of the second flow channel is configured to make a second material sprayed therefrom move towards a first material out of the outlet of the first flow channel, such that the second material sprayed from the outlet of the second flow channel and the first material sprayed from the outlet of the first flow channel are combined together to form a fluid printing unit. The bioprinter spray head assembly can guide the auxiliary materials to wrap the cells for protection, thereby reducing the cell damage caused by the extrusion pressure and the frictional force subjected in the printing process, so as to improve the survival rate of the cells and present a high reliability.

Abstract: The invention relates to the technical filed of tissue engineering and 3D printing, particularly relates to an artificial tissue progenitor and a method for preparing the same.

Abstract: The present disclosure relates to a microfluidic chip and a control method thereof, a droplet generation device and a microsphere preparation device. The microfluidic chip includes a matrix (3), and a first flow channel (1) and a second flow channel (2) provided in the matrix (3), wherein the first flow channel (1) and the second flow channel (2) intersect to form an intersection area, sheared phase fluid can flow in from the first flow channel (1), shearing phase fluid can flow in from the second flow channel (2) so as to separate the sheared phase fluid into discrete droplets in the intersection area, and the cross-sectional areas of the first flow channel (1) and the second flow channel (2) range from 0.1 mm2 to 1 mm2. The microfluidic chip can increase the flow rate and improve the efficiency of forming droplets; and the efficiency of generating the droplets is increased on the basis of ensuring the cell activity in order to meet the requirements of 3D biological printing.

Abstract: The present disclosure relates to a device for printing a lumen tissue construct, a method for using the same, and a 3D bioprinter. The device includes a spray head assembly for printing a biological construct; and a bioprinting platform for supporting a lumen tissue, and for carrying a biological construct printed by the spray head assembly, and for applying the biological construct to an inner surface of the lumen tissue. The device for printing a lumen tissue construct of the present disclosure provides the spray head assembly and the bioprinting platform, and the spray head assembly applies the biological construct onto the inner surface of the lumen tissue by the bioprinting platform, to avoid such problems as recurrence of thrombus and restenosis of a lumen after the lumen tissue has been implanted for a long time, thereby improving the biological reliability of the lumen tissue.

Abstract: A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.

Abstract: The present invention relates to the technical field of a bioprinter, and in particular relates to a bioprinter temperature control system and a bioprinter. The bioprinter temperature control system provided by the present invention, comprises a flow channel temperature control system, for controlling a temperature of a flow channel between an outlet of a bioprinting material container of a bioprinter and a nozzle of the bioprinter, such that the temperature of the flow channel conforms to a desired temperature of a biological printing material. The temperature control system of the present invention can realize the temperature control of the biological printing material, improving the survival rate of the printing material, and ensuring the biological function of the printing material.

Abstract: The present disclosure relates to a bioprinter spray head assembly and a bioprinter, wherein the bioprinter spray head assembly comprises an outer nozzle having a second channel and an inner nozzle having a first channel, the inner nozzle is coaxially provided within the second channel, the first channel forms a first material channel, and an annular space between the outer nozzle and the inner nozzle forms a second material channel, which surrounds the first material channel at an outlet of the first material channel, for converging a second material sprayed from an outlet of the second material channel toward a first material sprayed from the outlet of the first material channel, so as to form a fluid printing unit.

Abstract: The present invention relates to a rotary rod for 3D bio-printing, in which the rotary rod is arranged horizontally and is driven to rotate, the rotary rod has a hollow structure and provided with at least one hole in a surface thereof, such that during a 3D bio-printing process, a nutrition solution passes through the hollow structure and a portion of the nutrition solution exudes via at least one hole. The present invention further provides a 3D bio-printing platform for supplying nutrition, comprising the rotary rod and a nutrition supply system, and a method of printing a tubular tissue using the bio-printing platform. The present invention, which reduces the possibility of resulting in tissue collapse from the effect of gravity, provides a new method of 3D bio-printing a tubular tissue and supplying nutrition in a printing process, with a wide application prospect.

Abstract: A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.

Abstract: The present disclosure relates to a manufacturing method of a lumen tissue construct. The manufacturing method of a lumen tissue construct comprises: step 1: installing elastic film on a support in a sleeving manner; step 3: printing a biological construct on the elastic film; step 5: forming a construct bonding layer on the biological construct; and step 7: bonding a lumen tissue to the construct bonding layer. In the manufacturing method of the lumen tissue construct of the present disclosure, the printed biological construct is assembled on the inner surface of the lumen tissue to form the lumen tissue construct, thereby helping to avoid occurrence of problems such as recurrence of thrombus, restenosis of lumen and the like after the lumen tissue construct is implanted for a long time, and improving the biological reliability of the lumen tissue construct.

Abstract: A device and method for printing lumen tissue constructs and a 3D bioprinter are disclosed. The device has a spray sprayhead assembly and a bioprinting platform. The spray sprayhead assembly prints a biological construct on an inner surface of a lumen tissue by the bioprinting platform. Problems, such as, recurrence of thrombus and restenosis of a lumen after the lumen tissue has been transplanted for a long time, can be avoided. The biological reliability of the lumen tissue can be improved.

Abstract: The present disclosure provides a bio-ink cartridge, a bio-ink cartridge assembly, a microsphere preparation device, a shell assembly device, a bio-block preparation instrument, a bio-ink preparation instrument and a bio-ink preparation system. The bio-ink cartridge comprises an ink cartridge shell with a cavity; a dividing wall arranged in the cavity and dividing the cavity into a first accommodating chamber and a second accommodating chamber independently; a first ink cartridge inlet and a first ink cartridge outlet which are arranged on the ink cartridge shell, communicated with the first accommodating chamber and separated from the second accommodating chamber; a second ink cartridge inlet and a second ink cartridge outlet which are arranged on the ink cartridge shell and communicated with the second accommodating chamber; and an inner flow channel located in the second accommodating chamber and communicated with the first accommodating chamber and the first ink cartridge outlet.

Abstract: The present disclosure provides a bioprinter spray head assembly and a bioprinter, wherein the spray head assembly is internally provided with a first flow channel and a second flow channel, an outlet of the first flow channel is adjacent to an outlet of the second flow channel, and the outlet of the second flow channel is configured to make a second material sprayed therefrom move towards a first material out of the outlet of the first flow channel, such that the second material sprayed from the outlet of the second flow channel and the first material sprayed from the outlet of the first flow channel are combined together to form a fluid printing unit. The bioprinter spray head assembly can guide the auxiliary materials to wrap the cells for protection, thereby reducing the cell damage caused by the extrusion pressure and the frictional force subjected in the printing process, so as to improve the survival rate of the cells and present a high reliability.

Abstract: A device and method for printing lumen tissue constructs and a 3D bioprinter are disclosed. The device has a spray sprayhead assembly and a bioprinting platform. The spray sprayhead assembly prints a biological construct on an inner surface of a lumen tissue by the bioprinting platform. Problems, such as, recurrence of thrombus and restenosis of a lumen after the lumen tissue has been transplanted for a long time, can be avoided. The biological reliability of the lumen tissue can be improved.

Abstract: The present disclosure relates to a bioprinter spray head assembly, comprising a mounting block having a passage and a spray head mounted in the passage, wherein a first flow channel is provided in the mounting block, one end of the first flow channel is provided with a port communicating with the passage; the spray head comprises an equal diameter portion away from an outlet of the passage and a reduced diameter portion close to the outlet of the passage, wherein a second flow channel is formed between the reduced diameter portion and the passage, an opening is formed between the reduced diameter portion and the port to communicate the first flow channel and the second flow channel, and the equal diameter portion is configured to occlude the port. The adjustment of the circulation area of the spray head assembly may be achieved by changing the relative positions of the reduced diameter portion and the port, so that the adjustment is convenient and easy to carry out, and there is a favorable implementability.