Abstract: A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.

Abstract: Disclosed are aerosol delivery devices for use with a removable and replaceable cartridge, where the device includes an improved mouthpiece, such as one that is removable or customizable. The device includes a holder defining a receiving chamber configured to receive a removable cartridge comprising an ignitable heat source and a substrate portion that includes a substrate material having an aerosol precursor composition configured to form an aerosol upon application of heat thereto and a first aerosol passageway and a mouthpiece with a first end and a longitudinally opposed second end defining a second aerosol passageway, where the first end is configured to engage with a user's mouth and the second end is coupleable to the proximal end of the holder. The mouthpiece can be removed for cleaning and then reattached for continued use or, in some cases, a different mouthpiece may be attached to customize the user's experience.

Abstract: A connection device for fastening two expanded cellular confinement structures includes: an insertion member; an integral shank extending perpendicular from the insertion member; and an integral handle member extending perpendicular from the shank at an end of the shank remote from the insertion member. One or more barbs may project from the insertion member. The device can have a washer section between the handle member and insertion member. The handle can be curved, or it can be in the shape of a polygon.

Abstract: The invention provides a multi-layered smokeless tobacco product comprising two or more formulations with varying properties. In some embodiments, the smokeless tobacco products provide combinations of different organoleptic properties within the same product. In certain embodiment, these combinations allow for a modified release profile of active ingredients as the user enjoys the smokeless tobacco product. The invention further provides methods for making and using the smokeless tobacco product.

Abstract: An initiator assembly that includes a housing, a base, an exploding foil initiator, an input charge assembly and an output charge. The housing and the base cooperate to define a cavity. The exploding foil initiator and the output charge are received into the cavity. The input charge assembly is received in the cavity between the exploding foil initiator and the output charge. The input charge assembly includes a holder, which has first and second axial ends, and an input charge. A charge aperture is formed through the first axial end of the holder and does not extend through the second axial end of the holder. The input charge is formed of an explosive material and is received into the charge aperture.

Abstract: Methods and compositions are provided for expressing an endolysin in a bacterium or in a tobacco plant or plant part. Expression of the endolysin can reduce the total bacterial load on the plant and in tobacco products produced from the plant. Accordingly, the level of tobacco specific nitrosamines (TSNA) in a cured tobacco product can be decreased when the tobacco leaves used in the product were harvested from a plant expressing endolysin or when bacteria modified to express endolysin is used to treat the tobacco. Thus, bacteria and tobacco plants and plant parts are provided that express an endolysin. Further provided are methods for decreasing the microbial load on a tobacco plant and in a tobacco product by applying a solution of bacteria modified to express an endolysin or by modifying a tobacco plant to express endolysins. Smokeless tobacco products including such modified tobacco plant parts are also provided.

Abstract: A smokeless tobacco product is configured for insertion into the mouth of a user and incorporates materials from Nicotiana species (e.g., tobacco-derived materials) and plant materials from non-Nicotiana species (e.g., non-tobacco plant materials). The tobacco material can have the form of processed tobacco material (e.g., granulated, reconstituted, heat treated, or otherwise processed tobacco laminae and/or stem), tobacco extract (e.g., an extract of water soluble tobacco components obtained by extracting tobacco with water), or a combination thereof. The non-tobacco material can have the form of vegetable pulp (e.g., sugar beet pulp), pulp obtained after removal of water soluble components as a result of water extraction treatment, or a combination thereof.

Abstract: The invention provides certain nicotine salt co-crystals and provides novel polymorphic forms of certain nicotine salts. In particular, certain nicotine salt-co-crystals are described, including nicotine and two different coformers. The invention further provides methods of preparation and characterization of nicotine salts, co-crystals, and salt co-crystals. In addition, tobacco products, including smoking articles, smokeless tobacco products, and electronic smoking articles comprising nicotine salts, co-crystals, and/or salt co-crystals are also provided.

Abstract: A system for portioning a beaded substrate in a rod. The system includes a suction conveyor belt. The suction conveyor belt includes a belt suction chamber, a first belt end, and a second belt end, the second belt end opposite and downstream of the first belt end. The system also includes a metering device configured to provide the beaded substrate to the suction conveyor belt, and more specifically to the first belt end. The metering device includes a reach and a hopper. The metering device may provide the beaded substrate to the first belt end prior to the suction conveyor belt biasing a filler material onto the first belt end. The system may further includes a cutting mechanism that cooperates with the metering device to position the beaded substrate a first distance away from a first cut end.

Abstract: The present disclosure provides protein-enriched, tobacco-containing products, suitable for use as oral formulations. Products of the present disclosure typically include at least one tobacco material (e.g., a particulate tobacco material or a tobacco-derived extract), at least one protein-enriched material (e.g., a tobacco-derived protein-enriched material), and at least one sugar alcohol.

Abstract: An apparatus is provided for forming a cigarette filter rod member defining a longitudinal axis. A rod-forming unit is configured to form a continuous supply of a filter material into a continuous cylindrical rod member. An insertion unit configured to insert a carrier carrying a plurality of frangible microcapsule objects into the rod member. Associated apparatuses and methods are also provided.

Abstract: Methods and compositions are provided for expressing an endolysin in a bacterium or in a tobacco plant or plant part. Expression of the endolysin can reduce the total bacterial load on the plant and in tobacco products produced from the plant. Accordingly, the level of tobacco specific nitrosamines (TSNA) in a cured tobacco product can be decreased when the tobacco leaves used in the product were harvested from a plant expressing endolysin or when bacteria modified to express endolysin is used to treat the tobacco. Thus, bacteria and tobacco plants and plant parts are provided that express an endolysin. Further provided are methods for decreasing the microbial load on a tobacco plant and in a tobacco product by applying a solution of bacteria modified to express an endolysin or by modifying a tobacco plant to express endolysins. Smokeless tobacco products including such modified tobacco plant parts are also provided.

Abstract: A recloseable zipper pouch includes a recloseable zipper with interlocking profiles. At least one of the profiles has a grasping flange with a free end positionable both above and below the interlocking profile, and an attachment flange below the interlocking profile and attached to the wall of the pouch at an anchored portion on the attachment flange. The grasping flange is unattached to the first wall between opposite sidewall edges of the pouch. The complementary interlocking profile is attached to the opposite pouch wall. Some pouches can also include an opening in a side wall of the pouch to provide access to a portion of the grasping flange.

Abstract: A zipper closure includes mating first and second profiles, which releasably interlock along a parting line along the side. A slider can be used to open and close the slider. The slider has a channel, which receives an ear on the second profile, and the ear rides through the ear-receiving channel to align engaging structure of the first and second profiles and roll them together into interlocked engagement. The slider has a splitter on a deflectable tab, which can be pressed into a notch on the zipper closure to initially separate the mating profiles, while moving the slider to direct the ear into an ear-receiving channel in the slider and deflect the ear to a side and pry open and separate the profiles.

Abstract: The present disclosure is directed to various packaging devices or arrangements and the blanks configured to form the inner and outer packages that make up the devices. The blanks typically include a plurality of panels that are folded and bonded together to define a compartment when formed into a package. The compartment may be employed to store a consumable product, such as cartridges for an aerosol delivery device. Related methods for forming the blanks into the packages are also provided.

Abstract: A package that may be employed to store a tobacco-containing material is provided. The package may include a container including a body portion and a cover configured to engage the body portion. The body portion may define an internal space therein configured to receive a product. An internal surface of one or both of the body portion and the cover may include a wood liner. The wood liner may be a wood veneer.

Abstract: An overhead lighting fixture containing both visible light sources and UV light sources is disclosed herein. UV light can sanitize/disinfect the surrounding environment after the environment over a period of about 30-45 minutes. In this way, viruses, germs, bacteria, mold, and the like can be destroyed to clean the air and/or surfaces (e.g., furniture). Accordingly, embodiments of the present invention provide a controllable UV lighting assembly that can produce UV light to sanitize/disinfect an area and can be automatically disabled to prevent UV light exposure to those in the area using a motion sensor. The lighting assembly can produce visible light while the UV light source is disabled. According to some embodiments, the duration of UV light production is controlled by a timer for power savings.

Abstract: A firing circuit for operating an exploding foil initiator that employs a transistor to control the transmission of electrical energy from an electrical energy storage device to a bridge of the exploding foil initiator.

Abstract: A smoking article is provided, having opposed lighting and mouth ends, and including a mouth end portion at the mouth end. A tobacco portion is between the lighting end and the mouth end portion. An aerosol-generation system is between the lighting end and the tobacco portion. The aerosol-generation system includes a heat generation portion, comprising an elongate fluted member actuated by ignition of the lighting end. The fluted member defines grooves extending longitudinally between opposed first and second ends, with the first end being at the lighting end and the grooves being equidistantly spaced apart about the fluted member. Each groove has a maximum depth. The depth maxima of the grooves define a circle having a radius. The maximum depth of each groove is no more than the radius of the circle. A heat generation apparatus for an aerosol-generation system of a smoking article is also provided.