Patents Assigned to RGENE, INC.
  • Multiple ligase compositions, systems, and methods
    Patent number: 11124789
    Abstract: Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient ATP-dependent ligase or an un-adenylated ATP-dependent ligase (e.g., present in an ATP free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e.g., the adenylation-deficient ATP-dependent ligase or un-adenylated ATP-dependent ligase ligates the first strand, and a second ligase ligates the second strand). In other embodiments, provided herein are mutant T4 ligases (e.g., K159S mutant or K159C mutant).
    Type: Grant
    Filed: April 1, 2020
    Date of Patent: September 21, 2021
    Assignee: RGENE, Inc.
    Inventors: Yu Zheng, Manqing Hong
  • Multiple ligase compositions, systems, and methods
    Patent number: 10626390
    Abstract: Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient ATP-dependent ligase or an un-adenylated ATP-dependent ligase (e.g., present in an ATP free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e.g., the adenylation-deficient ATP-dependent ligase or un-adenylated ATP-dependent ligase ligates the first strand, and a second ligase ligates the second strand). In other embodiments, provided herein are mutant T4 ligases (e.g., K159S mutant or K159C mutant).
    Type: Grant
    Filed: February 7, 2017
    Date of Patent: April 21, 2020
    Assignee: RGENE, INC.
    Inventors: Yu Zheng, Manqing Hong