Abstract: Provided in the present invention are a separated endogenous rice gene resistant to high temperature (hereinafter referred to as the OsZFP gene for short) and a polypeptide encoded thereby, optimizing rice cells comprising the heat-resistant gene of the present invention or the polypeptide encoded thereby, and the plant cell preparation method thereof. Further provided are new methods and technologies for breeding new varieties of heat-resistant crops, comprising the related regulatory sequence for heat-resistance and a closely linked molecular marker denoting the heat-resistant gene and the sequence thereof.