Abstract: A fluorometric luminescence immunoassay method includes forming a sample by exposing a first immune reaction reactant to a second immune reaction reactant capable of reacting with the first reactant, one of the first and second immune reaction reactants being labelled with a photoluminescent energy transfer donor and the other being labelled with a photoluminescent energy transfer acceptor complementary to the photoluminescent donor. At least the photoluminescent donor has the property of photoluminescence, and the photoluminescent donor and acceptor are chosen so that when the first immune reaction reactant reacts with the second immune reaction reactant, the donor and the acceptor are capable of interacting to produce a detectable luminescence lifetime change. The sample is excited with radiation, and the resulting emission is detected. The apparent luminescent lifetime is then calculated to determine the presence of a reaction product of the first and second immune reaction reactants.

Abstract: A tamper evident dual snap on cap and spout for a plastic bottle neck has a removable first closure having an upper skirt and an upstanding spout with an inwardly spaced sealing flange. A tear skirt joined to the lower most edge of the upper skirt with a circumferential tear line and depending tab extending therefrom. A second closure has an annular band engageable on a portion of the bottle neck, an inwardly spaced sealing flange and a pour spout engageable with the upstanding spout and inwardly spaced sealing flange of the first closure. Removal of the tear skirt allows removal of the first closure and access to the closure's pour spout.