Abstract: A method for assessing if an individual or an animal has a selected condition includes: obtaining a biological sample from the individual or animal; detecting in a time-resolved manner the presence of a disease related target through use of a probe (111); calculating a multidimensional fingerprint (141) which represents the recorded binding curves (131); extracting a predefined region (151) or feature of the distribution; and using the region or feature to determine (170) if the individual or animal has the selected condition. The method is particularly advantageous for tissue slices combined with antibody probes, the antibody recognizing receptors known to be over-expressed in cancer. In even more particular, a method for the assessment of HER2 expression level in breast cancer is described.

Abstract: A method of analysis or diagnosis of solid objects is based on real-time detection of how predefined probes interact with structures present on or in the solid object combined with the calculation of how the recorded binding curves of said probes are distributed in terms of interaction properties. The interaction properties are input to a classification algorithm which automatically determines statues of the solid object. The method is particularly advantageous for solid biological objects like tissue slices combined with antibody probes, said antibody recognizing receptors known to be over-expressed in disease states on said tissue slice.