Abstract: The invention relates to recombinant host cells that expresses one or more genes encoding a cytochrome P450 enzyme capable of N-demethylating and/O-demethylating reticuline and/or derivatives thereof, and also methods of producing a N-demethylated and/or O-demethylated reticuline and/or derivatives thereof, comprising cultivating the recombinant host of the invention in a culture medium under conditions in which the one or more genes encoding the cytochrome P450 enzymes is/are expressed. The reticuline and derivatives thereof are useful for providing access to naturally unavailable and chemically difficult-to-produce starting materials for opioids.
Type:
Grant
Filed:
June 18, 2018
Date of Patent:
October 10, 2023
Assignee:
RIVER STONE BIOTECH, LLC
Inventors:
Markus Schwab, Sumire Honda Malca, Philipp Friedrich Berninger, Jon Richard Heal, Joseph Michael Sheridan, Laura Tatjer Recorda, Rubini Maya Kannangara, Esben Halkjaer Hansen, Angela Manuela Ribeiro de Carvalho
Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.
Type:
Grant
Filed:
August 9, 2017
Date of Patent:
July 13, 2021
Assignee:
RIVER STONE BIOTECH, LLC
Inventors:
Esben Halkjaer Hansen, Markus Schwab, Philipp Berninger, Fanny Delgrange, Franziska Grassinger