Abstract: A novel composition for treating cancer comprising of a gRNA-Cas9 complex capable of locating, binding, and causing a double-strand break of the gene that is prevented from repair by accompanying DNA repair inhibitors.

Abstract: TNF-alpha (Tumor Necrosis Factor-alpha) is a cytokine, a protein involved in the inflammation process in the body. It plays a crucial role in the immune system, primarily produced by activated macrophages the white blood cells. TNF-alpha is critical in the inflammatory response to infections and diseases, but it can also contribute to inflammatory and autoimmune diseases when produced in excess. Many antibodies are created to inhibit over-expressed TNF-alpha but with serious side effects. The present invention provides a mRNA vaccine to reduce the titer of TNF-alpha, leading to treating multiple life-threatening diseases including but not limited to rheumatoid arthritis, psoriatic arthritis, Crohn's disease, ankylosing spondylitis, plaque psoriasis, inflammatory bowel disease, type 2 diabetes, tumor progression, angiogenesis, and metastasis, heart failure from myocardial cell death, and atherosclerosis from fibrosis.

Abstract: mRNA coding vaccines to treat neurodegenerative disorders (NDs), particularly Alzheimer's Disease (AD), Amyotrophic Lateral Sclerosis (ALS), Corticobasal Degeneration (CBD), Frontotemporal Dementia (FTD), Lewy Body Dementia (LBD), Multiple System Atrophy (MSA), Parkinson's Disease Dementia (PSD), and Progressive Supranuclear Palsy (PSP), to scavenge improperly folded or aggregated proteins 2MXU (PDB) (A?42), P10636 TAU_HUMAN (Tau Protein), P37840·SYUA_HUMAN (?-Synuclein), Q13148.TADBP_HUMAN (TDP-43), and P35637·FUS_HUMAN by generating specific antibodies based on the epitopes of these proteins. The epitopes can be linked to generate the open reading frames of mRNA vaccines.

Abstract: An mRNA composition for malaria prevention and treatment comprising a coding mRNA capable of expressing proteins and peptides of malaria pathogens, coming from linear epitopes and protein structure of antigens.

Abstract: Darbepoetin alpha treats anemia and it is traditionally manufactured using recombinant Chinese Hamster Ovary cells, a complex and expensive process; this invention reports an mRNA composition that encodes darbepoetin alpha in vivo as a therapeutic protein.

Abstract: The present invention relates to a composition for expressing botulinum toxin in target tissue by injecting or topically applying an mRNA product capable of expressing the toxin.