Patents Assigned to Roche Diagnostics GmbH
  • Method for generating multiple double stranded nucleic acids
    Patent number: 6326143
    Abstract: The present invention concerns a method for generating multiple double stranded nucleic acids by (a) elongating a primer molecule comprising a nucleobase sequence B′ by using one or more nucleotide(s) and a target nucleic acid T that acts as a template for the elongation of said primer such that the elongation product E formed is capable of acting as a template for the elongation of a further primer molecule containing the nucleobase sequence B′; (b) separating the target nucleic acid T from said elongation product E; (c) using said elongation product E as a template for the elongation of a further primer molecule yielding an elongation product E′; and (d) repeating the steps of elongating primer molecules and of separating said elongation products a sufficient number of times to achieve the desired amount of double stranded nucleic acid.
    Type: Grant
    Filed: May 22, 1998
    Date of Patent: December 4, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Henrik Ørum, Corina Seeger
  • Use of thiadiazolo[4,3-a]pyridine derivatives
    Patent number: 6326378
    Abstract: The present invention relates to the method of treating leukemia, lymphoma or inhibiting the proliferation of cells with a compound of formula I.
    Type: Grant
    Filed: October 5, 2000
    Date of Patent: December 4, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Walter-Gunar Friebe, Wolfgang Schaumann, Otto-Henning Wilhelms
  • Boehringer Mannheim
    Publication number: 20010046714
    Abstract: Subject matter of the invention is a reagent preparation for binding components of a sample in the form of a tablet comprising a multitude of magnetic particles which are held together with the aid of excipients, and the use of this reagent preparation in analytical test,
    Type: Application
    Filed: June 29, 2001
    Publication date: November 29, 2001
    Applicant: Roche Diagnostics GmbH
    Inventor: Herbert Harttig
  • Method for the determination of lipase
    Patent number: 6322993
    Abstract: The invention concerns a method and reagent for the determination of lipase in the presence of a colour substrate and at least one N-substituted carboxylic acid amide derivative or a compound of the general formula (I) or (Ia) in which R1 and R2 independently of one another represent hydrogen or a saturated or unsaturated, substituted or unsubstituted hydrocarbon residue with 2 to 24 carbon atoms, Z denotes a saturated or unsaturated, substituted, cyclic or straight-chained hydrocarbon residue with 1 to 10 carbon atoms, X represents an atom or a group of atoms with positive charge and n is a number from 1 to 3. Tetra-sodium-N-(1,2-dicarboxylethyl)-N-alkylsulfosuccinamide or a mixture containing such a compound has proven to be particularly advantageous for the elimination of unspecific reactions in the determination of lipase in a biological sample.
    Type: Grant
    Filed: May 8, 1998
    Date of Patent: November 27, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Lieselotte Schelong, Ralf Zielenski, Urban Prinzing
  • C-nucleoside derivatives and their use in the detection of nucleic acids
    Patent number: 6320035
    Abstract: The invention concerns pyrrolo-[3,2-d]pyrimidine, pyrazolo-[4,3-d]pyrimidine and pyrimidine-furanosides i.e. so-called C-nucleosides of the general formulae I-V or appropriate derivatives as well as processes for their production.
    Type: Grant
    Filed: October 25, 2000
    Date of Patent: November 20, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Klaus Mühlegger, Herbert Von Der Eltz, Frank Seela, Helmet Rosemeyer
  • Recombinant inactive core streptavidin mutants
    Patent number: 6312916
    Abstract: The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.
    Type: Grant
    Filed: April 1, 1997
    Date of Patent: November 6, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Erhard Kopetzki, Rainer Müller, Richard Engh, Urban Schmitt, Arno Deger, Hans Brandstetter
  • 2-alkylidene hydroxycoumaranone derivatives
    Patent number: 6307051
    Abstract: New compounds of formula I wherein: R and R1 are independently selected from hydrogen, (C1-C6)alkyl, styryl and (C3-C6)cycloalkyl or, taken together with the carbon to which they are linked, form a (C3-C6)cycloalkyl group; A is selected from the following groups: —CH2C≡CCH2—, or —(CH2)q—NH—(CH2)q—, wherein q is an integer from 2 to 3 B is selected from T is selected from —CH2—C≡CH, —C≡CH, —(CH2)p—R3, —CH═CH—R3, —CH2—NHCO—R3, —(CH2)p—O—R3, —CH(NH2)—CH2R3, in which p is 0 or an integer from 1 to 4, R3 is a carbocyclic or heterocyclic ring as medicaments having antitumor and/or antimetastatic activities.
    Type: Grant
    Filed: January 31, 2000
    Date of Patent: October 23, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Walter-Gunar Friebe, Bernhard Koenig, Hans-Willi Krell, Sabine Woelle
  • Recombinant antigen from the NS3 region of the hepatitis C virus
    Patent number: 6306579
    Abstract: The present invention concerns a polypeptide which is composed of the amino acids 1207±10 to 1488±10 of a hepatitis C virus and of less than 20 foreign amino acids and the use of this polypeptide as an antigen in an immunological test.
    Type: Grant
    Filed: July 15, 1997
    Date of Patent: October 23, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Christoph Seidel, Ursula-Henrike Wienhues, Urban Schmitt, Manfred Motz, Michael Wiedmann, Barbara Upmeier, Erwin Soutschek
  • Method for quantification of an analyte
    Patent number: 6303305
    Abstract: The invention provides a new method for the quantification of a nucleic acid, wherein in a first step, the nucleic acid is amplified by an amplifying agent. Subsequently, first, second or n th order derivatives is calculated. The obtained value can then be used to calculate the initial concentration of the analyte.
    Type: Grant
    Filed: March 30, 1999
    Date of Patent: October 16, 2001
    Assignees: Roche Diagnostics, GmbH, University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Martin Gutekunst, Sabine Lohmann
  • Process for the production of pharmaceutical preparations containing human protein for infusion or injection purposes
    Patent number: 6303113
    Abstract: The present invention concerns a process for the production of well-tolerated, preserved injection or infusion solutions containing human protein.
    Type: Grant
    Filed: January 30, 1996
    Date of Patent: October 16, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Heinrich Woog, Werner Gruber, Hans-Jörg Markl, Gerhard Winter, Fritz Demmer
  • Host-vector system
    Patent number: 6291245
    Abstract: A prokaryotic expression vector including an origin of replication, at least one eukaryotic auxotrophy marker gene which encodes an enzyme required for the synthesis of a product necessary for the survival of at auxotrophic prokaryote under the control of a eukaryotic promoter, a foreign gene under the control of a prokaryotic promoter, and one or more transcription terminators.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: September 18, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Erhard Kopetzki, Christian Schantz
  • Asymmetric porous membranes
    Patent number: 6287867
    Abstract: The invention concerns the use of an asymmetric porous membrane for the evenly distributed concentration of a substance which is not or not substantially adsorbed to the membrane and which is brought into contact with the membrane in the form of a membrane-wetting solution on the fine-pored side of the membrane.
    Type: Grant
    Filed: August 26, 1994
    Date of Patent: September 11, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Herbert Harttig, Hartmut Merdes, Hans Lange, Manfred Zeiler
  • Process for the production of proteins in soluble form by modulation of an inducible promoter
    Patent number: 6284484
    Abstract: The present invention provides a process for the expression in appropriate host cells of recombinant DNA which is under the control of an inducible promotor. In order to obtain the soluble form of recombinant protein, which would usually be obtained in insoluble form, the induction of the promotor is limited to less than 10% of the maximum induction in comparison with a standard system and the transcription rate of the DNA coding for the protein is correspondingly limited.
    Type: Grant
    Filed: June 27, 1991
    Date of Patent: September 4, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Erhard Kopetzki, Günther Schumacher
  • Positive-negative selection for homologous recombination
    Patent number: 6284541
    Abstract: The invention concerns a method for the introduction of a foreign DNA into the genome of a target cell by homologous recombination as well as for the homologous recombination of suitable DNA constructs.
    Type: Grant
    Filed: June 9, 2000
    Date of Patent: September 4, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Johannes Auer, Raimund Sprenger, Konrad Honold
  • Use of derivatized reagents in chemiluminescence and electrochemiluminescence detection methods
    Patent number: 6281021
    Abstract: The invention concerns methods for the detection of an analyte in a sample by chemiluminescence or electrochemiluminescence using derivatized test reagents. In addition new reagents and reagent kits for chemiluminescence and electrochemiluminescence detection methods are disclosed.
    Type: Grant
    Filed: June 18, 1999
    Date of Patent: August 28, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Martin Egger, Hans-Peter Josel, Gabriele Punzmann
  • Recombinant blood-coagulation proteases
    Patent number: 6277618
    Abstract: The invention relates to a non-glycosylated protein with enzymatic and serin protease activity, the zymogenous form thereof comprising the following domains of a protease of the factor IX family: (a) a catalytic domain, N-terminal bonded with (b) a zymogenous activation domain, N terminal bonded with (c) a EGF1 and/or EGF2 domain. Said protein can be used in a same way as the natural serine proteases of the factor IX family.
    Type: Grant
    Filed: April 12, 1999
    Date of Patent: August 21, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Erhard Kopetzki, Karl-Peter Hopfner
  • Reagent preparation containing magnetic particles in tablet form
    Patent number: 6274386
    Abstract: Subject matter of the invention is a reagent preparation for binding components of a sample in the form of a tablet comprising a multitude of magnetic particles which are held together with the aid of excipients, and the use of this reagent preparation in analytical test.
    Type: Grant
    Filed: June 6, 1997
    Date of Patent: August 14, 2001
    Assignee: Roche Diagnostics GmbH
    Inventor: Herbert Harttig
  • Apparatus with desiccant chamber and method of using
    Patent number: 6274304
    Abstract: There is provided a system for the preparation of liquids from at least one solid and at least one liquid phase. The system is composed of a vessel in which the solid or solids are located and a drying chamber which is connected to the vessel or can be connected to the vessel. The drying chamber is separated from the vessel interior by a separating means which is permeable to water vapour but is impermeable to liquids. The system is suitable for the production of liquids from moisture-sensitive solids. These solids can be stored in the vessel since the air humidity in the interior is lowered by the desiccant. The preparation of the liquid by addition of a liquid phase can be carried out in one and the same vessel since the desiccant is separated from the vessel interior.
    Type: Grant
    Filed: February 14, 1994
    Date of Patent: August 14, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Herbert Buschek, Michael Kuehnl, Roland Ihrig, Klaus Erler, Maria Cully
  • Method and reagent for the interference-free determination of iron
    Patent number: 6274382
    Abstract: Method and reagent for the determination of iron in a biological sample in which the bound iron is released, the released iron is reduced to Fe2+, a color reagent solution is added and the color complex that is formed is measured photometrically, characterized in that a water-soluble EDTA-complexing compound in particular an indium and/or scandium salt is added to the sample.
    Type: Grant
    Filed: April 22, 1999
    Date of Patent: August 14, 2001
    Assignee: Roche Diagnostics GmbH
    Inventor: Wolfgang Treiber
  • Process for producing recombinant proteins using a gene for tRNA
    Patent number: 6270988
    Abstract: The present invention provides a process for the expression of a recombinant gene, which contains AGA and/or AGG codons for arginine, in Escherichia coli after transformation with an expression vector which contains the recombinant gene, wherein the amount of t-RNA present in the E. coli cells which incorporates arginine and recognises the codons AGG and AGA is increased to at least fivefold of the amount normally occurring in these cells.
    Type: Grant
    Filed: January 27, 1993
    Date of Patent: August 7, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Ulrich Brinkmann, Ralf Mattes, Stephan Fischer