Abstract: The invention is directed to an oligonucleotide comprising a molecular rod, wherein preferentially the molecular rod covalently connects the 3? end of a first nucleotide residue with the 5? end of a second nucleotide residue. Such oligonucleotides are highly advantageous for real time PCR melting curve analysis.
Type:
Grant
Filed:
December 2, 2004
Date of Patent:
June 2, 2009
Assignee:
Roche Diagnostis Operations, Inc.
Inventors:
Dieter Heindl, Astrid Reiser, Karim Tabiti, Martina Junius, Alexander Hauber