Patents Assigned to Rohm Enzyme Finland Oy
  • Patent number: 6667170
    Abstract: The production of thermostable xylanses having bacterial origin is described. These compositions are useful for modifying plant biomass and for enzyme-aided bleaching of wood pulp.
    Type: Grant
    Filed: January 22, 1999
    Date of Patent: December 23, 2003
    Assignee: Röhm Enzyme Finland Oy
    Inventors: Arja Mäntylä, Marja Paloheimo, Raija Lantto, Richard Fagerström, Tarja Lahtinen, Pirkko Suominen, Jari Vehmaanperä
  • Patent number: 6635464
    Abstract: DNA encoding novel xylanases, vectors containing such DNA, hosts transformed with such DNA, enzyme preparations, and the use of such preparations are described.
    Type: Grant
    Filed: May 7, 2001
    Date of Patent: October 21, 2003
    Assignee: Rohm Enzyme Finland OY
    Inventors: Marja Paloheimo, Satu Hakola, Arja Mantyla, Jari Vehmaanpera, Raija Lantto, Tarja Lahtinen, Richard Fagerström, Pirkko Suominen
  • Publication number: 20030148453
    Abstract: The present invention is related to an improved production of bacterial proteins in filamentous fungus, e.g. in Tfichodenna and Aspergillus. The improvement is achieved by constructing expression vectors, which comprise the bacterial protein encoding DNA sequences fused in frame with a DNA sequence encoding a filamentous fungus secretable protein or one or more functional domains of said protein. Filamentous fungus hosts transformed with such expression vectors secrete the desired proteins or enzymes, especially xylanases or cellulases originating from bacteria or more preferably from actinornycetes into the culture medium of the host. The desired proteins or enzymes can be used directly from the culture medium after separation of host cells or recovered and treated using down-stream processes, which are appropriate for the respective application. Xylanases or cellulases from actinomycetes produced using the above expression vectors are most suitable for treating plant derived materials e.g.
    Type: Application
    Filed: August 13, 2002
    Publication date: August 7, 2003
    Applicant: Rohm Enzyme Finland Oy
    Inventors: Arja Mantyla, Marja Paloheimo, Raija Lantto, Richard Fagerstrom, Tarja Lahtinen, Pirkko Suominen, Jari Vehmaanpera
  • Patent number: 6506593
    Abstract: The production of thermostable xylanses having bacterial origin is described. These compositions are useful for modifying plant biomass and for enzyme-aided bleaching of wood pulp.
    Type: Grant
    Filed: January 29, 2001
    Date of Patent: January 14, 2003
    Assignee: Rohm Enzyme Finland OY
    Inventors: Arja Mäntylä, Marja Paloheimo, Raija Lantto, Richard Fagerström, Tarja Lahtinen, Pirkko Suominen, Jari Vehmaanperä
  • Patent number: 6300114
    Abstract: The production of thermostable xylanses having bacterial origin is described. These compositions are useful for modifying plant biomass and for enzyme-aided bleaching of wood pulp.
    Type: Grant
    Filed: January 26, 1996
    Date of Patent: October 9, 2001
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Aria Mäntylä, Marja Paloheimo, Raija Lantto, Richard Fagerström, Tarja Lahtinen, Pirkko Suominen, Jari Vehmaanperä
  • Patent number: 6184019
    Abstract: Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
    Type: Grant
    Filed: June 10, 1999
    Date of Patent: February 6, 2001
    Assignee: Röhm Enzyme Finland OY
    Inventors: Arja Miettinen-Oinonen, John Londesborough, Jari Vehmaanperä, Heli Haakana, Arja Mäntylä, Raija Lantto, Minna Elovainio, Vesa Joutsjoki, Marja Paloheimo, Pirkko Suominen
  • Patent number: 6011147
    Abstract: A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: January 4, 2000
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Tiina Hannele Nakari, Maija-Leena Onnela, Marja Hannele Ilmen, Kaisu Milja Helena Nevalainen, Merja Elisa Penttila
  • Patent number: 5989870
    Abstract: A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.
    Type: Grant
    Filed: February 16, 1995
    Date of Patent: November 23, 1999
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Tiina Hannele Nakari, Maija-Leena Onnela, Marja Hannele Ilmen, Merja Elisa Penttila
  • Patent number: 5935836
    Abstract: The isolation and cloning of Actinomadura spp. xylanases having a molecular weight of 35 kDa and 50 kDa are described. These xylanases are thermostable and useful in biobleaching of wood pulp.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: August 10, 1999
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Jari Vehmaanpera, Arja Mantyla, Richard Fagerstrom, Raija Lantto, Marja Paloheimo, Pirkko Suominen
  • Patent number: 5922579
    Abstract: Enzyme compositions containing thermostabile xylanases of Chaetomium thermophilum, purified enzyme preparations of such xylanases, and the use of such compositions and preparations in the bleaching of plant pulp and in feed and baking applications are described.
    Type: Grant
    Filed: December 17, 1996
    Date of Patent: July 13, 1999
    Assignee: Rohm Enzyme Finland OY
    Inventors: Richard B. Fagerstrom, Marja Paloheimo, Raija Lantto, Tarja Lahtinen, Pirkko Suominen
  • Patent number: 5874293
    Abstract: The present invention is directed to a new cellulase composition for treating and finishing cellulose-containing textiles. The improved properties of the cellulase composition are based on an elevated content of EGII endoglucanase type component in an otherwise complete cellulase composition. When the composition with elevated EGII contents is used improved color properties, increased lightness, improved visual appearance and reduced pilling tendencies are achieved. The strength properties of the textile materials are essentially unchanged as compared to previously used cellulase compositions.
    Type: Grant
    Filed: January 31, 1997
    Date of Patent: February 23, 1999
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Arja Sisko Kaarina Miettinen-Oinonen, Minna Johanna Elovainio, Pirkko Liisa Suominen
  • Patent number: 5858767
    Abstract: The present invention is directed to a new cellulase composition for finishing of cellulose-containing textiles, especially cotton. The improved properties of the cellulase composition are based on an elevated content of CBHII cellobiohydrolase type component in an otherwise normal background cellulase composition. When the composition with elevated CBHII contents is used improved color properties, increased lightness, improved visual appearance and reduced pilling tendencies are achieved. The strength properties of the textile materials are essentially unchanged as compared to previously used cellulase compositions.
    Type: Grant
    Filed: January 31, 1997
    Date of Patent: January 12, 1999
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Arja Sisko Kaarina Miettinen-Oinonen, Minna Johanna Elovainio, Pirkko Liisa Suominen
  • Patent number: 5854047
    Abstract: The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing.
    Type: Grant
    Filed: August 13, 1997
    Date of Patent: December 29, 1998
    Assignee: Rohm Enzyme Finland OY
    Inventors: Johanna Buchert, Matti Siika-aho, Liisa Viikari, Merja Penttila, Anu Saloheimo, Marjatta Ranua
  • Patent number: 5834286
    Abstract: The present invention provides a recombinant combination strain which is capable of over-expressing at least two different genes under two separate promoters in filamentous fungi. The genes encode phytase and pH 2.5 acid phosphatase. Mixtures containing desired ratios of the two enzymes are prepared by recombinant DNA techniques. The enzyme mixtures show a cooperative effect in the degradation of phytic acid and its salts. The preferred ratios of the two enzymes are from about 3:1 to about 16:1.
    Type: Grant
    Filed: May 24, 1995
    Date of Patent: November 10, 1998
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Helena K. M. Nevalainen, Marja T. Paloheimo, Richard B. Fagerstrom, Arja S. K. Miettinen-Oinonen, Marja K. Turunen, John A. Rambosek, Christopher S. Piddington, Christine S. Houston, Michael A. Cantrell
  • Patent number: 5830733
    Abstract: The present invention provides a nucleic acid molecule encoding a phytase. The present invention also provides a nucleic acid molecule encoding a pH 2.5 acid phosphatase. Also provided are vectors, host cells, and a method of overexpressing phytate degrading enzymes.
    Type: Grant
    Filed: March 1, 1996
    Date of Patent: November 3, 1998
    Assignee: Rohm Enzyme Finland Oy
    Inventors: Helena K. M. Nevalainen, Marja T. Paloheimo, Arja S. K. Miettinen-Oinonen, Tuula K. Torkkeli, Michael Cantrell, Christopher S. Piddington, John A. Rambosek, Marja K. Turunen, Richard B. Fagerstrom, Christine S. Houston