Abstract: A method and apparatus for conducting specific binding assays are claimed. This invention relates to an initial non-receptor ligand interaction with nanoparticles or other solid phases followed by specific detection methods used for detecting biological, chemical or environmental entities. This invention employs an on-device ligand attachment to nanoparticles or other solid phase followed by the specific capture in discrete zones on the device. The ligand-bound complexes assemble on the ligand specific capture zone leading to a visible diagnostic result, if colored solid phases are employed.

Abstract: A method and apparatus for conducting specific binding assays with two or more particles is claimed. A non-magnetic nanoparticle capable of binding the target of interest is mixed with a magnetic particle capable of binding a species directed toward binding the same target of interest. Following the application of the mixture to a capillary tube or other container that allows the separation with a magnetic field or other force, the resultant movement of the magnetic or combination of magnetic and non-magnetic assemblies can be read visually or with the aid of an appropriate instrument.