Abstract: Methods of preparing and using a highly active blood coagulation factor XI mutant and a gene therapy/editing vector thereof and a recombinant/fusion protein thereof. The nucleotide sequence of the mutant is as shown in SEQ ID NOs: 1-6, and the amino acid sequence is as shown in SEQ ID NO: 7.

Abstract: A high-activity blood coagulation factor XI mutant Ala570Thr (A570T), having nucleotide sequences as shown in SEQ ID NOs: 1-4 and an amino acid sequence as shown in SEQ ID NO: 5, is provided. The mutant is resistant to a physiological inhibitor thereof after being activated from a zymogen state to an active enzyme. Therefore, the mutant has a very high blood coagulation activity and a stronger catalytic ability for a non-physiological substrate; and the mutant is applied to the treatment of hemorrhagic diseases, and has good prospects in terms of gene therapy, gene editing and recombinant protein replacement treatments.

Abstract: Methods of preparing and using a highly active blood coagulation factor XI mutant and a gene therapy/editing vector thereof and a recombinant/fusion protein thereof. The nucleotide sequence of the mutant is as shown in SEQ ID NOs: 1-6, and the amino acid sequence is as shown in SEQ ID NO: 7.

Abstract: An infusion pump comprises a housing (1); and a controller (3), a direct current motor (4), a transmission mechanism (6), a rotary encoder (5), and a liquid reservoir (7), which are located within the housing (1), wherein: a piston (71) is provided within the liquid reservoir (7); the transmission mechanism (6) has one end connected with the piston (71) and the other end connected with the direct current motor (4); the rotation shaft of the rotary encoder (5) is connected with the rotation shaft (41) of the direct current motor (4); the controller (3) is connected with the rotary encoder (5) and is provided thereon with a built-in clock and an input key (2) capable of receiving an external control signal. The infusion pump can freely adjust the infusion time and the infusion dose.