Abstract: Separation of all clinically relevant isoamylase bands including P.sub.1 P.sub.1b, P.sub.2, P.sub.3, P.sub.4, S.sub.1, S.sub.2, S.sub.3, S.sub.4 and S.sub.5 is achieved in a test in which a biological sample is electrophoresed on an agarose gel supporting a tris-sodium barbital-barbital buffer at a pH of between 8.4 and 9.2. The barbital anion concentration is between about 0.03 and about 0.08 M, the tris cation concentration is between about 0.03 M and about 0.07 M; and the sodium cation concentration is between about 0.03 M and about 0.07 M. Agarose is present in the gel at concentrations of between about 0.4 and about 1.5 weight percent.

Abstract: A sterile preparation of synthetic erythrocytes consisting of hemoglobin fraction encapsulated within water-immiscible amphiphylic membranes provides a total hemoglobin of at least about 12 gm percent at a hematocrit of 50%. A lipid composition is prepared and dispersed by agitation in a sterile, stroma-free 30-45 gram percent hemoglobin fraction. The dispersion is pressurized to between about 400 to about 900 kg/cm.sup.2, and the pressure is substantially instantaneously released by passing the mixture to a lower pressure region through an orifice having an area of between about 0.1 and about 10.0 mm.sup.2, thereby forming the synthetic erythrocytes. The preparation is filtered through a filter which passes particles having a diameter less than about 0.22 micron to eliminate any monocellular organisms that may be present and to eliminate larger synthetic erythrocytes.

Abstract: An apparatus and method are provided for producing signals representative of the mean cell volume of red blood cells in a blood specimen. The apparatus includes means for generating signals repesentative of the area of the cells and means for measuring the optical density of the individual cells and for generating signals representative of the hemoglobin content or mass of the cells. The central pallor is measured for cells having central pallors and means generate a signal representative of the central pallor of these cells. The representative area signals, hemoglobin signals and central pallor signals are sent to a means which generates an output representative of the mean cell volume of the cells.

Abstract: A method and apparatus for determining and displaying hemodynamic characteristics of a living body instantaneously and over a time period, i.e., rate of blood flow, stroke volume, cardiac output, cardiac index, systemic vascular resistance, useful power of the heart and elastic properties of the walls of blood vessels. The improved method and apparatus deal with physical measurements of body electrical impedance and blood pressure in relationship with other known or derived factors to produce signals representing these characteristics and which may be displayed for the information of surgeons or others who may be interested in this information.

Abstract: Chondrocytes are cultured to produce cartilage tissue from which an anti-invasion factor (AIF) may be recovered. Mammalian cartilage providing at least about 60% viable chondrocytes is enzymatically digested to denude the cells of their extracellular matrix. The denuded cells are plated at high density and cultured to produce cartilage tissue.

Abstract: A femoral insert for a hip joint prosthesis wherein the proximal and distal portions are angularly joined to define a lateral apex, the apical height of which has a particular ratio to the medial length of the insert.

Abstract: An apparatus for preparing blood samples containing red blood cells for automated analysis wherein the blood sample on a slide is spun to create a monolayer of randomly distributed red blood cells. To inhibit cell morphology distortions from occurring during drying, the morphologies of the cells contained in the monolayer are preserved by a fixing agent after monolayer preparation but prior to drying when such distortions would otherwise develop. The method and apparatus are particularly useful for fixing red blood cells to prevent loss of or deformation of a central pallor which would be detrimental to a subsequent automated analysis.

Abstract: A method for preparing blood samples containing red blood cells for automated analysis wherein the blood sample on a slide is spun to create a monolayer of randomly distributed red blood cells. To inhibit cell morphology distortions from occurring during drying, the morphologies of the cells contained in the monolayer are preserved by a fixing agent after monolayer preparation but prior to drying when such distortions would otherwise develop. The method and apparatus are particularly useful for fixing red blood cells to prevent loss of or deformation of a central pallor which would be detrimental to a subsequent automated analysis.

Abstract: A method and apparatus are disclosed for measuring characteristics of cells, such as red blood cells, and for analyzing parameters of the cell characteristics to define a patient's blood. These parameters may be compared for resemblance to predetermined reference characteristic values for a blood cell pathological condition such as a specific kind of anemia or for a normal blood. A report may be generated showing such resemblance to an anemia or to a normal blood. A report may be generated showing parameters of a multivariate dispersion of distribution for a subpopulation of biconcave cells, an indication of skewness of the distribution of the cells with regard to shape variations in central pallor size; the proportion of abnormal kinds of cells found, and closeness of blood to several specific anemias.

Abstract: A method for regulating bone resorption wherein the bone is exposed to an effective amount of a composition of matter obtained from avascular or poorly vascularized tissue by extracting the tissue with an aqueous extraction medium which includes a solute which does not irreversibly denature the proteinaceous matter to be extracted and recovering from the aqeuous extract substances having molecular weight below about 50,000.

Abstract: A method and apparatus is disclosed for use in performing automated classification of cells of a pap smear as well as other types of cells, such as red blood cells and blood cell neutrophiles. The apparatus generates an image that is split into two paths, (1) a high resolution image of primarily the nucleus of a cell, and (2) a low resolution image of the total cell, nucleus and cytoplasm. An image scanning device located in the first path provides output signals representing the image and circuitry performs measurement and analysis relating to texture of the nucleus. The image in the other path is demagnified which provides an enlarged, lower resolution image of the entire cell, including the nucleus and the cytoplasm and it is projected to a second image scanning device, which provides electrical signals that represent the image of the larger area and circuitry performs measurement and analysis relating to the size, density and color of the cytoplasm and the nucleus.

Abstract: A method and apparatus for automatically classifying abnormal and normal red blood cells is disclosed. The abnormal cells may be differentiated into mutually exclusive subpopulations. The individual hemoglobin characteristic for each red blood cell is measured and a hemoglobin parameter may be reported for each subpopulation of cells. Additionally, cell sizes are measured and the mean cell size and Wintrobe indices may be reported. The interior cell structures for red blood cells are analyzed and the cells are classified based on the presence or lack of a central pallor, their shape, size, and hemoglobin content.

Abstract: A composition of matter having activity as an inhibitor of cell proliferation is obtained by aqueous extraction of tissue having a high content of collagen and/or proteoglycans.