Abstract: A gas mixer and sampler system (2) for a controlled atmosphere laboratory chamber (4), the mixer and sampler system has a sampler remote from the chamber and a gas supply arrangement, including a mixer (14) remote from the chamber. The sampler consists of a gas sensor arrangement (5) for sensing a first and a second gas. A processor output from the gas sensors to determine the relative concentrations of the first and second gases, and determine proportions and flow rate for gases to be supplied to the chamber to meet a determined profile. The mixer and sampler are provided externally of the chamber at a location spaced from the chamber.

Abstract: A controlled environment system for use in In-Vitro Fertilization (IVF) or other biological procedures and a method for conducting such a biological procedure. The system includes a main chamber, one or more manipulator devices for manipulating items in the interior of the chamber, a microscopy viewing system enabling viewing of the manipulation procedure, means for modifying the proportions of gas in a multi gas chamber atmosphere set up in the main chamber, chamber heater means for heating the gas atmosphere in the main chamber, a recirculation gas circuit providing for recirculation of the main chamber gas atmosphere, the recirculation gas circuit including a particulate filter arrangement and a sterilization arrangement.