Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.
Type:
Grant
Filed:
March 9, 2023
Date of Patent:
March 12, 2024
Assignee:
S2 GENOMICS, INC.
Inventors:
Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
Abstract: This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.
Type:
Grant
Filed:
January 23, 2022
Date of Patent:
August 8, 2023
Assignee:
S2 GENOMICS, INC.
Inventors:
Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.
Type:
Grant
Filed:
June 1, 2019
Date of Patent:
April 4, 2023
Assignee:
S2 GENOMICS, INC.
Inventors:
Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced.
Type:
Grant
Filed:
October 28, 2021
Date of Patent:
September 13, 2022
Assignee:
S2 GENOMICS, INC.
Inventors:
Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced.
Type:
Grant
Filed:
November 29, 2017
Date of Patent:
January 25, 2022
Assignee:
S2 GENOMICS, INC.
Inventors:
Stevan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira