Abstract: A centrifuge layering insert configured for insertion into a centrifuge tube includes a tube having a first open end and a second open end. The centrifuge layering insert also includes a casing having an outer wall, an inner wall, a first open end, and a second substantially closed end. The casing forms an annular space around the tube between the tube first end and the tube second end. The tube has at least a portion of the length extending beyond the first open end of the casing in a direction opposite the second open end of the tube.

Abstract: The present invention is directed towards an apparatus and method for disinfecting biological samples, including semen and the like, and for removing a biological sample pellet from surrounding media while reducing the risk of recontamination of the pellet with said media. An insert receptacle includes a receiving cylinder having a drain in a floor which allows the fluids to decant slowly down the side of a tube. The insert receptacle further includes an aspiration channel which allows an aspiration device, such as a modified micro-pipette, to be utilized for extracting a sample pellet from the bottom of the tube. Utilizing the novel apparatus, a method for reducing pathogens in a biological sample includes decanting the biological sample through the receiving cylinder and into a tube where it passes through a multi-layer gradient including an enzyme suitable for removal of at least one pathogen from the biological sample.

Abstract: The present invention is directed to a method and apparatus for disinfection of biological samples, including semen and the like. In a first aspect of the present invention, a method for reducing pathogens in a biological sample includes passing the biological sample through a gradient including an enzyme suitable for removal of at least one pathogen from the sample. In an additional aspect of the present invention, an apparatus for removing pathogens from a biological sample includes a container including a gradient having at least one layer that is suitable for having the biological sample pass through the layer, the at least one layer including an enzyme suitable for removing at least one pathogen from the biological sample.