Patents Assigned to Sage Science, Inc.
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Patent number: 11867661Abstract: An electrophoresis cassette may include sample well(s), gel column(s) containing a separation gel, and elution modules arranged adjacent the gel column(s). A sample may be provided to the electrophoresis cassette and high-molecular weight (HMW) DNA may be isolated from the sample. Single-copy DNA sequences may be cleaved on both sides of a repeat region of the DNA sequences to produce a cleaved sample, which then may be fractionated using gel electrophoresis. DNA fractions may be isolated from consecutive sections of the separation gel and subjected to PCR assays to detect single-copy sequences within the DNA fraction, said single-copy sequence containing repeat expansion sequences. The subjected DNA fractions may be electroeluted into the plurality of elution modules. A size of DNA fractions having the repeat expansion sequences may be determined. It is also determined if that size is above a normal repeat size range.Type: GrantFiled: April 6, 2018Date of Patent: January 9, 2024Assignee: SAGE SCIENCE, INC.Inventor: T. Christian Boles
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Publication number: 20230227808Abstract: A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase re-agents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.Type: ApplicationFiled: January 3, 2023Publication date: July 20, 2023Applicants: Sage Science, Inc., Washington UniversityInventors: Robi David Mitra, Jeffrey MILBRANDT, Ezra Solomon ABRAMS, Todd J. BARBERA, T. Christian BOLES
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Patent number: 11542495Abstract: A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.Type: GrantFiled: November 21, 2016Date of Patent: January 3, 2023Assignees: Sage Science, Inc., Washington UniversityInventors: Robi David Mitra, Jeffrey Milbrandt, Ezra Solomon Abrams, Todd J. Barbera, T. Christian Boles
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Publication number: 20220106549Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.Type: ApplicationFiled: October 19, 2021Publication date: April 7, 2022Applicant: SAGE SCIENCE, INC.Inventor: Gary Paul MAGNANT
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Publication number: 20210207122Abstract: A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.Type: ApplicationFiled: November 21, 2016Publication date: July 8, 2021Applicants: Sage Science, Inc., Washington UniversityInventors: Robi David MITRA, Jeffrey MILBRANDT, Ezra Solomon ABRAMS, Todd J. BARBERA, T. Christian BOLES
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Patent number: 10738298Abstract: Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.Type: GrantFiled: November 15, 2018Date of Patent: August 11, 2020Assignee: SAGE SCIENCE, INC.Inventors: Ezra S. Abrams, Danny Yun, Todd J. Barbera, Douglas Grosvenor Sabin, T. Christian Boles
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Publication number: 20200087607Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.Type: ApplicationFiled: November 25, 2019Publication date: March 19, 2020Applicant: SAGE SCIENCE, INC.Inventor: Gary Paul MAGNANT
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Patent number: 10519411Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.Type: GrantFiled: February 8, 2017Date of Patent: December 31, 2019Assignee: Sage Science, Inc.Inventor: Gary Paul Magnant
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Patent number: 10473619Abstract: The disclosure provides cassettes, electrophoresis systems, methods for making the device, and methods of fractionating a sample using the cassettes and electrophoresis systems described herein.Type: GrantFiled: March 20, 2017Date of Patent: November 12, 2019Assignee: SAGE SCIENCE, INC.Inventors: Douglas Grosvenor Sabin, Joshua Gomes, Todd J. Barbera, Charles Sidoti, Simranjit Singh, T. Christian Boles
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Publication number: 20190153426Abstract: Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.Type: ApplicationFiled: November 15, 2018Publication date: May 23, 2019Applicants: SAGE SCIENCE, INC., SAGE SCIENCE, INC.Inventors: Ezra S. ABRAMS, Danny YUN, Todd J. BARBERA, Douglas Grosvenor SABIN, T. Christian BOLES
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Patent number: 10131901Abstract: Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.Type: GrantFiled: October 15, 2015Date of Patent: November 20, 2018Assignee: SAGE SCIENCE, INC.Inventors: Ezra S. Abrams, Danny Yun, Todd J. Barbera, Douglas Grosvenor Sabin, T. Christian Boles
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Patent number: 9719961Abstract: The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.Type: GrantFiled: June 15, 2016Date of Patent: August 1, 2017Assignee: SAGE SCIENCE, INC.Inventors: Douglas Grosvenor Sabin, Todd J. Barbera, Paul Chandler Sabin, T. Christian Boles
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Publication number: 20170145366Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.Type: ApplicationFiled: February 8, 2017Publication date: May 25, 2017Applicant: SAGE SCIENCE, INC.Inventor: Gary Paul MAGNANT
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Patent number: 9597355Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.Type: GrantFiled: March 14, 2014Date of Patent: March 21, 2017Assignee: SAGE SCIENCE, INC.Inventor: Gary Paul Magnant
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Patent number: 9599590Abstract: The disclosure provides cassettes, electrophoresis systems, methods for making the device, and methods of fractionating a sample using the cassettes and electrophoresis systems described herein.Type: GrantFiled: October 10, 2013Date of Patent: March 21, 2017Assignee: Sage Science, Inc.Inventors: Douglas Grosvenor Sabin, Joshua Gomes, Todd J. Barbera, Charles Sidoti, Simranjit Singh, T. Christian Boles
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Patent number: 9012373Abstract: Systems and methods for processing fluid samples are disclosed. Fluid sample processing is accomplished using a series of microfluidic bump arrays include an automated and integrated system for sorting particles from a biological sample, lysing those particles to expose total RNA or DNA, purifying the RNA or DNA, processing the RNA or DNA by chemical or enzymatic modification, to select RNA or DNA molecules by size, or to generate, optionally, a sequencing library. The sequencing library is suitable for use in next generation sequencing (“NGS”).Type: GrantFiled: August 3, 2012Date of Patent: April 21, 2015Assignee: Sage Science, Inc.Inventors: T. Christian Boles, Ezra Solomon Abrams
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Publication number: 20140271571Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.Type: ApplicationFiled: March 14, 2014Publication date: September 18, 2014Applicant: Sage Science, Inc.Inventor: Gary Paul Magnant
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Publication number: 20130240360Abstract: The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.Type: ApplicationFiled: January 28, 2013Publication date: September 19, 2013Applicant: SAGE SCIENCE, INC.Inventors: Douglas Grosvenor Sabin, Todd J. Barbera, Paul Chandler Sabin, T. Christian Boles
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Publication number: 20130233714Abstract: The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.Type: ApplicationFiled: January 28, 2013Publication date: September 12, 2013Applicant: SAGE SCIENCE, INC.Inventors: Douglas Grosvenor Sabin, Todd J. Barbera, Paul Chandler Sabin, T. Christian Boles
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Publication number: 20130079251Abstract: Systems and methods for processing fluid samples are disclosed. Fluid sample processing is accomplished using a series of microfluidic bump arrays include an automated and integrated system for sorting particles from a biological sample, lysing those particles to expose total RNA or DNA, purifying the RNA or DNA, processing the RNA or DNA by chemical or enzymatic modification, to select RNA or DNA molecules by size, or to generate, optionally, a sequencing library. The sequencing library is suitable for use in next generation sequencing (“NGS”).Type: ApplicationFiled: August 3, 2012Publication date: March 28, 2013Applicant: Sage Science, Inc.Inventor: T. Christian Boles