Abstract: Provided is a method of manufacturing a drug delivery system including a first surfactant and a second surfactant, the drug delivery system manufactured by using the method, and a contrast medium, wherein the first surfactant includes a polyethylene glycol based nonionic surfactant and the second surfactant includes a Tween-type surfactant or a SPAN-type surfactant. According to the present invention, the microbubble including the first surfactant, which includes a polyethylene glycol (PEG) based nonionic surfactant, and the second surfactant, which includes a Tween-type surfactant or a SPAN-type surfactant, may stably have uniform size distribution, may include high loading amount of drugs and dye on the surface of the delivery system, and thereby, may be efficiently used as a drug delivery system. Also, drug release may increase by ultrasonic wave, which is external stimulus, from the microbubble and thereby, more efficient drug delivery may be available.

Abstract: An ultrasound-induced drug delivery system is described, using a drug carrier containing a plurality of nanobubbles and a high concentration of a drug in one microcapsule, and a method for preparing the drug delivery system, by generating the nanobubbles in an oil into which the drug is dissolved using a nanobubble generator, and then microencapsulating them. The drug delivery system has an effect of maximizing a drug delivery efficiency as the nanobubbles collapse or aggregate when the ultrasound is applied to the drug delivery system. Since the drug delivery system contains a plurality of nanobubbles within the microcapsules, it can also be used as a contrast agent, or can be used to simultaneously perform in vivo diagnosis and treatment.

Abstract: Proposed are an ultrasound-assisted drug delivery carrier containing a ligand linked with a drug through an ester bond, a phospholipid, and a PEGylated phospholipid, a composition for drug delivery including the drug delivery carrier, and a method of preventing or treating a disease including administering the composition to an individual other than a human and releasing a drug by subjecting the site of administration of the composition to ultrasound irradiation. The ultrasound-assisted drug delivery carrier containing a ligand linked with a drug through an ester bond, a phospholipid, and a PEGylated phospholipid can be provided in the form of microbubbles or nanobubbles, and is capable of accelerating drug release due to collapse of the bubbles and promotion of hydrolysis of the ester bond during ultrasound irradiation, making it possible to deliver the drug to a desired site with high efficiency.

Abstract: The present invention relates to a bifidobacterium adolescentis SPM0212 having antiviral activity for hepatitis B virus, and a pharmaceutical composition for preventing and treating hepatitis B comprising thereof. The bifidobacterium adolescentis SPM0212 or its fraction of the present invention shows antiviral activity for hepatitis B virus by blocking the production of antigen s of hepatitis B virus, and by inhibiting the expression of DNA, RNA and HBsAg of the HBV.

Abstract: The present invention relates to a bifidobacterium adolescentis SPMO212 having antiviral activity for hepatitis B virus, and a pharmaceutical composition for preventing and treating hepatitis B comprising thereof. The bifidobacterium adolescentis SPMO212 or its fraction of the present invention shows antiviral activity for hepatitis B virus by blocking the production of antigen s of hepatitis B virus, and by inhibiting the expression of DNA, RNA and HBsAg of the HBV.