Abstract: A method for generating amplicon constructs of a target sequence is disclosed, the method comprising providing a target sequence; an oligonucleotide probe, comprising a universal sequence and further comprising, at or towards its 5? end, a target specific sequence capable of hybridising to the reverse complement of a sequence at, or flanking one of the 3? ends of the target sequence; a universal primer, comprising at its 3? end a sequence capable of hybridising to the universal sequence of the oligonucleotide probe and performing a Polymerase Chain Reaction (PCR).

Abstract: A method for generating amplicon constructs of a target sequence is disclosed, the method comprising providing a target sequence; an oligonucleotide probe, comprising a universal sequence and further comprising, at or towards its 5? end, a target specific sequence capable of hybridising to the reverse complement of a sequence at, or flanking one of the 3? ends of the target sequence; a universal primer, comprising at its 3? end a sequence capable of hybridising to the universal sequence of the oligonucleotide probe and performing a Polymerase Chain Reaction (PCR).

Abstract: A shoe insole has a cavity configured to provide an internal pocket open to a face of said insole, conveniently the lower face, the cavity having a length greater than its width and being directed generally along the length of the insole. A segmented structure is slideably insertable into said cavity within it is removably retained, the segments being disposed along the cavity and housing a pressure sensor, sensor state monitoring circuits, a wireless transceiver and a battery, adjacent segments being electrically connected together and hinged together by pairs of laterally spaced connector bars each mechanically connected together by links configured to maintaining the spacing between segments while permitting flexion between adjacent segments under loads encountered during walking.

Abstract: A controller is provided for a functional electrical stimulator for attachment to a leg comprising, a connector for a foot switch for sensing foot rise or foot strike, a circuit for responding to said foot switch for generating stimulation pulses and a connector for first and second electrodes for attachment to the leg for supplying stimulation pulses from said circuit The circuit includes a voltage divider of which the foot switch when connected comprises one element, a second element being provided by a digital potentiometer forming part of the controller. A micro controller is configured to make adaptive adjustment of the resistance of said digital potentiometer to take account of the resistance characteristics of the foot switch to provide an output or reference voltage permitting an open/closed state of the switch to be monitored.

Abstract: A controller is provided for a functional electrical stimulator for attachment to a leg comprising, a connector for a foot switch for sensing foot rise or foot strike, a circuit for responding to said foot switch for generating stimulation pulses and a connector for first and second electrodes for attachment to the leg for supplying stimulation pulses from said circuit The circuit includes a voltage divider of which the foot switch when connected comprises one element, a second element being provided by a digital potentiometer forming part of the controller. A micro controller is configured to make adaptive adjustment of the resistance of said digital potentiometer to take account of the resistance characteristics of the foot switch to provide an output or reference voltage permitting an open/closed state of the switch to be monitored.