Abstract: Methods and systems are provided herein for monitoring pathogens in various environments and on various items, wherein data from monitoring is trackable, analyzable and comparable versus various standards or thresholds. The methods and systems disclosed herein also include a platform for managing the detection and reporting of pathogens across a number of locations within a number of environments, and using such detection for a wide variety of purposes.

Abstract: This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.