Abstract: The present application relates to methods for determining the concentration of one or more hormones in a sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Abstract: The systems and methods described herein provide a SmartTube collection and logging technique of a patient's (or clinical trial participant/subject) action of self-collection of a biologic sample during a sample collection event. The system for recording the sample collection event may include a shipping container comprising a sensor area, a switch and a microcontroller and sample collector positioned on the sensor area of the shipping container. The removal of the sample collector from the sensor area can change a state of the switch to a first state and the return of the sample collector to the sensor area can change the state of the switch to a second state during the sample collection event. The microcontroller can be coupled to the switch to receive a signal from the switch indicating the change in state.