Abstract: The present invention provides a method of determining a lung cancer treatment, the method comprising: measuring an amount of KL-6 in a sample of body fluid from a subject, and determining the treatment based on the amount of KL-6 measured, and a method of determining the effectiveness of an agent for treatment of lung cancer on a subject, the method comprising: measuring an amount of KL-6 in a sample of body fluid from the subject, determining the effectiveness of an EGFR inhibitor on the subject should the EGFR inhibitor be administered as the agent for treatment of lung cancer, based on the amount of KL-6 measured.

Abstract: The present invention is intended to devise a process of measuring ?-amyloid in a biological sample such as blood and to apply the process to diagnosis of Alzheimer's disease. It is possible to assay Alzheimer's disease by measuring a total amount of ?-amyloid 1-42 and ?-amyloid 1-42 fragments each of which retains a C-terminal site of the ?-amyloid 1-42 in the biological sample by an immunological assay in which an antibody which recognizes the C-terminal site of ?-amyloid 1-42 is used. It is preferable that the immunological assay be a competitive immunological assay.

Abstract: Provided are a plasma or serum separator and a plasma or serum sampling method capable of isolating plasma or serum with good efficiency from a small amount of blood without using a centrifuge and without causing leakage of a blood cell component or hemolysis, and in addition, capable of isolating and collecting plasma or serum from a whole blood test sample in a short time with simplicity in a blood test in the scene of medical care requiring an instant treatment any time such as an emergency test, home-use test or the like.

Abstract: There is provided a method for forming a self-assembly substance using oligonucleotides without using special instruments or complicated procedures, an self-assembly substance formed by the method for forming the self-assembly substance, and a method for detecting an amplified target gene at a low cost and in a simple way by making use of the method for forming the self-assembly substance. In the method for forming the self-assembly substance using a self-assembly reaction of oligonucleotides, the oligonucleotides comprise oligonucleotides synthesized by a gene amplification reaction. The oligonucleotides synthesized by the gene amplification reaction are detected by forming a self-assembly substance by the use of the method for forming the self-assembly substance of oligonucleotides, and by detecting the formed self-assembly substance.

Abstract: The present invention provides a novel method for forming a self-assembly substance of probes making it possible to shorten a reaction time required for formation of a self-assembly substance and also to increase regions of probes available for detection of a target gene by previously preparing a plurality of dimer-probes and increasing the types. This method comprises the steps of: providing plural groups, wherein each group includes a pair of dimer forming probes containing a pair of oligonucleotides, each oligonucleotide having three regions of a 3? side region, a mid-region and a 5? side region, in which the mid-regions of the oligonucleotides have base sequences complementary to each other, and the 3? side regions and the 5? side regions of the oligonucleotides have base sequences not complementary to each other; hybridizing a plurality of pairs of the dimer forming probes of the plural groups; and forming a double-stranded self-assembly substance by self-assembly of the oligonucleotides.

Abstract: The present invention provides a method for measuring a target gene under isothermal conditions without using enzyme. A pair of probes each having n (n?3) base sequence regions complementary to each other are hybridized alternately to form a double-stranded probe-polymer. A base pair at branched sites of each complementary base sequence region is designed to be a G (guanine)-C (cytosine) bond, whereby a stable double-stranded probe-polymer is formed. One of complementary portions in one probe is constituted to have a base sequence complementary to a part of a target gene, whereby a target gene-probe-polymer complex is formed and the target gene is measured.

Abstract: The present invention provides an immunoassay for specifically measuring with high sensitivity PIVKA-II in serum or plasma through antigen-antibody reaction by adding an animal serum containing thrombin and/or an antibody reacting with human fibrin-like related substances to the reagents. The immunoassay of the invention comprises the steps of adding thrombin and/or an antibody reacting with human fibrin-like related substances to the reagents, and measuring PIVKA-II in serum or plasma.

Abstract: A gene amplifying method for efficiently amplifying a gene without using enzyme or branched DNA. A plurality of pairs of probes each composed of three or more portions complementary to such portions in the other probe are used, and hybridized such that they cross in alternation to form a double-stranded polymer.

Abstract: An automatic immunoassay method and apparatus which not only permit automatic operations of supplying and discarding reaction tubes, washing for B/F separation, dispensation of reagents, and measurement, but also permit the reaction time to be varied arbitrarily and can cope with any of the assay techniques including one-step and two-step noncompetitive sandwich techniques and competitive techniques.

Abstract: A method of easily detecting the occurrence of colorectal cancer in the absence of occult blood is provided by measuring decay accelerating factor (DAF) molecules which are synthesized by colorectal cancer cells and present in feces. The method includes reacting an anti-DAF antibody with a supernatant of a fecal solution and measuring an amount of the antibody which has bonded to DAF molecules by an antigen-antibody reaction with the DAF molecules in the supernatant.

Abstract: An antigen-antibody complex in which two molecules of IgG class monoclonal anti-D are bound to one or two molecules of monoclonal or polyclonal anti-IgG antibody specific to the IgG class monoclonal anti-D. It reacts with Rh(D) positive blood cells, causing an observable agglutination and is useful for quick Rh blood typing.